Anti-Phospho-FOXO4 (Ser262) Polyclonal Antibody 2 is a Rabbit antibody targeting Phospho-FOXO4 (Ser262). Anti-Phospho-FOXO4 (Ser262) Polyclonal Antibody 2 can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Rat (predicted:Mouse,Chicken,Dog,Pig,Cow,Horse)

1. Tissue/cell: Rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-AFX Polyclonal Antibody, Unconjugated (TMAB-10717) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
2. Sample: Hela (Human) Cell Lysate at 30 μg
Primary: Anti-phospho-FOXO4 (Ser262) (TMAB-10717) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56 kD
Observed band size: 56 kD

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated Synthesised phosphopeptide: human FOXO4 around the phosphorylation site of Ser262

Human

FOXO4

Forkhead box protein O4

Transcription factor involved in the regulation of the insulin signaling pathway. Binds to insulin-response elements (IREs) and can activate transcription of IGFBP1. Down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. Also involved in negative regulation of the cell cycle.