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Anti-Phospho-FOXO1 (Ser319) Polyclonal Antibody

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Catalog No. TMAB-01413
Alias p-FOXO1 (Ser319), p-FOXO1 (S319), Foxo1a, FOXO1A, FOXO1 (p-Ser319), FOXO1 (p-S319), Forkhead, Drosophila, homolog of, in rhabdomyosarcoma, Forkhead(Drosophila) homolog 1(rhabdomyosarcoma), Forkhead in rhabdomyosarcoma, Forkhead box protein O1A, Forkhead box protein O1, Forkhead box O1, Forkhead, Fkhr1, FKHR, FKH1, FKH 1, AI876417, Afxh

Anti-Phospho-FOXO1 (Ser319) Polyclonal Antibody is a Rabbit antibody targeting Phospho-FOXO1 (Ser319). Anti-Phospho-FOXO1 (Ser319) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-Phospho-FOXO1 (Ser319) Polyclonal Antibody

Anti-Phospho-FOXO1 (Ser319) Polyclonal Antibody

😃Good
Catalog No. TMAB-01413Alias p-FOXO1 (Ser319), p-FOXO1 (S319), Foxo1a, FOXO1A, FOXO1 (p-Ser319), FOXO1 (p-S319), Forkhead, Drosophila, homolog of, in rhabdomyosarcoma, Forkhead(Drosophila) homolog 1(rhabdomyosarcoma), Forkhead in rhabdomyosarcoma, Forkhead box protein O1A, Forkhead box protein O1, Forkhead box O1, Forkhead, Fkhr1, FKHR, FKH1, FKH 1, AI876417, Afxh
Anti-Phospho-FOXO1 (Ser319) Polyclonal Antibody is a Rabbit antibody targeting Phospho-FOXO1 (Ser319). Anti-Phospho-FOXO1 (Ser319) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack SizePriceAvailabilityQuantity
50 μL$2227-10 days
100 μL$3727-10 days
200 μL$5287-10 days
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Product Introduction

Bioactivity
Description
Anti-Phospho-FOXO1 (Ser319) Polyclonal Antibody is a Rabbit antibody targeting Phospho-FOXO1 (Ser319). Anti-Phospho-FOXO1 (Ser319) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Aliasp-FOXO1 (Ser319), p-FOXO1 (S319), Foxo1a, FOXO1A, FOXO1 (p-Ser319), FOXO1 (p-S319), Forkhead, Drosophila, homolog of, in rhabdomyosarcoma, Forkhead(Drosophila) homolog 1(rhabdomyosarcoma), Forkhead in rhabdomyosarcoma, Forkhead box protein O1A, Forkhead box protein O1, Forkhead box O1, Forkhead, Fkhr1, FKHR, FKH1, FKH 1, AI876417, Afxh
Ig Type
IgG
Reactivity
Human,Mouse,Rat (predicted:Chicken,Dog,Pig,Cow,Horse)
Verified Activity
1. Paraformaldehyde-fixed, paraffin embedded (rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Phospho-FoxO1 (Ser319)) Polyclonal Antibody, Unconjugated (TMAB-01413) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Phospho-FoxO1 (Ser319)) Polyclonal Antibody, Unconjugated (TMAB-01413) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded (mouse liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Phospho-FoxO1 (Ser319)) Polyclonal Antibody, Unconjugated (TMAB-01413) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded (mouse colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Phospho-FoxO1 (Ser319)) Polyclonal Antibody, Unconjugated (TMAB-01413) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Phospho-FoxO1 (Ser319)) Polyclonal Antibody, Unconjugated (TMAB-01413) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
6. Sample:
Lane 1: Lung (Rat) Lysate at 40 μg
Lane 2: Pancreas (Rat) Lysate at 40 μg
Lane 3: LympHnode (Rat) Lysate at 40 μg
Lane 4: Bone (Rat) Lysate at 40 μg
Primary: Anti-Phospho-FoxO1 (Ser319) (TMAB-01413) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 78 kDa
Observed band size: 75 kDa
7. Paraformaldehyde-fixed, paraffin embedded (mouse spleen); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Phospho-FoxO1 (Ser319)) Polyclonal Antibody, Unconjugated (TMAB-01413) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
8. Paraformaldehyde-fixed, paraffin embedded (rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Phospho-FoxO1 (Ser319)) Polyclonal Antibody, Unconjugated (TMAB-01413) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
9. Paraformaldehyde-fixed, paraffin embedded (rat colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Phospho-FoxO1 (Ser319)) Polyclonal Antibody, Unconjugated (TMAB-01413) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
10. Paraformaldehyde-fixed, paraffin embedded (rat spleen); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Phospho-FoxO1 (Ser319)) Polyclonal Antibody, Unconjugated (TMAB-01413) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
11. Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Phospho-FoxO1 (Ser319)) Polyclonal Antibody, Unconjugated (TMAB-01413) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
12. Tissue/cell: human cervical carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-Phospho-FoxO1 (Ser319) polyclonal Antibody, Unconjugated (TMAB-01413) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
Application
WB,IHC-P,IHC-Fr,IF
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationCytoplasm. Nucleus. Note=Shuttles between cytoplasm and nucleus. Translocates to the nucleus upon oxidative stress induced phosphorylation at Ser-212 by STK4/MST1. Translocates to the nucleus upon phosphorylation of Thr-24, Ser-256 and Ser-322 by SGK1.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
FormulationPreservative: 0.02% Proclin300, Constituents: 1% BSA, 0.01M PBS, pH7.4.
Research BackgroundThis gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. The specific function of this gene has not yet been determined; however, it may play a role in myogenic growth and differentiation. Translocation of this gene with PAX3 has been associated with alveolar rhabdomyosarcoma. [provided by RefSeq].
Antigen Details
Immunogen
KLH conjugated synthesised phosphopeptide: human FoxO1 around the phosphorylation site of Ser319
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
Coactivators and repressors,Co-activators/co-repressors,FOXO,Obesity,Neurogenesis,Co-activators/co-repressors
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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