Anti-Phospho-FHIT (Tyr114) Polyclonal Antibody 2 is a Rabbit antibody targeting Phospho-FHIT (Tyr114). Anti-Phospho-FHIT (Tyr114) Polyclonal Antibody 2 can be used in IHC-P,IHC-Fr,IF.

IgG

Human (predicted:Mouse,Rat,Dog,Horse,Rabbit)

Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-Phospho-FHIT (Tyr114) Polyclonal Antibody, Unconjugated (TMAB-10702) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining

IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated Synthesised phosphopeptide: human FHIT around the phosphorylation site of Tyr114

Human

FHIT

Bis(5'-adenosyl)-triphosphatase

Hydrolyzes glucose-6-phosphate to glucose in the endoplasmic reticulum. Forms with the glucose-6-phosphate transporter (SLC37A4/G6PT) the complex responsible for glucose production through glycogenolysis and gluconeogenesis. Hence, it is the key enzyme in homeostatic regulation of blood glucose levels.