Anti-Phospho-Estrogen Receptor beta (Ser87) Polyclonal Antibody is a Rabbit antibody targeting Phospho-Estrogen Receptor beta (Ser87). Anti-Phospho-Estrogen Receptor beta (Ser87) Polyclonal Antibody can be used in WB,ICC/IF,FCM.

IgG

Human,Mouse (predicted:Rat,Rabbit,Sheep)

1. Sample:
Lane 1: Testis (Mouse) Lysate at 40 ug
Lane 2: Ovary (Mouse) Lysate at 40 ug
Lane 3: Uterus (Mouse) Lysate at 40 ug
Primary: Anti-phospho-Estrogen Receptor beta (Ser87) (TMAB-10679) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 59 kD
Observed band size: 59 kD
2. 4% Paraformaldehyde-fixed MCF-7 (H) cell; Triton X-100 at r. T. for 20 min; Antibody incubation with (phospho-Estrogen Receptor beta (Ser87)) polyclonal Antibody, unconjugated (TMAB-10679) 1: 100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, FITC) at 37°C for 90 min, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
3. The MCF-7 (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-phospho-Estrogen Receptor beta (Ser87) antibody (TMAB-10679, 1: 100); Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed.

WB: 1:500-2000; ICC/IF: 1:50-200; FCM: 1μg/Test

Polyclonal

KLH conjugated synthesised phosphopeptide: human Estrogen Receptor beta around the phosphorylation site of Ser87

Human

ESR2

Estrogen receptor beta

Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues.