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Anti-Phospho-ERK1/2 (Thr202, Tyr204) Antibody (4P145)
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Catalog No. TMAY-01512
Alias PRKM3, Phospho-ERK1/2 (T202, Y204), p-ERK1/2 (Thr202, Tyr204), p-ERK1/2 (T202, Y204), p44-MAPK, P44MAPK, p44-ERK1, P44ERK1, HUMKER1A, HS44KDAP, ERT2, ERK-2, Erk2, ERK1/2 (p-Thr202, Tyr204), ERK1/2 (p-T202, Y204), ERK-1, ERK1
Anti-Phospho-ERK1/2 (Thr202, Tyr204) Antibody (4P145) is a Rabbit antibody targeting Phospho-ERK1/2 (Thr202, Tyr204). Anti-Phospho-ERK1/2 (Thr202, Tyr204) Antibody (4P145) can be used in WB.
Anti-Phospho-ERK1/2 (Thr202, Tyr204) Antibody (4P145)
Anti-Phospho-ERK1/2 (Thr202, Tyr204) Antibody (4P145)
😃Good
Catalog No. TMAY-01512Alias PRKM3, Phospho-ERK1/2 (T202, Y204), p-ERK1/2 (Thr202, Tyr204), p-ERK1/2 (T202, Y204), p44-MAPK, P44MAPK, p44-ERK1, P44ERK1, HUMKER1A, HS44KDAP, ERT2, ERK-2, Erk2, ERK1/2 (p-Thr202, Tyr204), ERK1/2 (p-T202, Y204), ERK-1, ERK1
Anti-Phospho-ERK1/2 (Thr202, Tyr204) Antibody (4P145) is a Rabbit antibody targeting Phospho-ERK1/2 (Thr202, Tyr204). Anti-Phospho-ERK1/2 (Thr202, Tyr204) Antibody (4P145) can be used in WB.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 20 μL | $154 | 7-10 days | |
| 100 μL | $450 | 7-10 days |
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| Description | Anti-Phospho-ERK1/2 (Thr202, Tyr204) Antibody (4P145) is a Rabbit antibody targeting Phospho-ERK1/2 (Thr202, Tyr204). Anti-Phospho-ERK1/2 (Thr202, Tyr204) Antibody (4P145) can be used in WB. |
| Synonyms | PRKM3, Phospho-ERK1/2 (T202, Y204), p-ERK1/2 (Thr202, Tyr204), p-ERK1/2 (T202, Y204), p44-MAPK, P44MAPK, p44-ERK1, P44ERK1, HUMKER1A, HS44KDAP, ERT2, ERK-2, Erk2, ERK1/2 (p-Thr202, Tyr204), ERK1/2 (p-T202, Y204), ERK-1, ERK1 |
| Ig Type | Rabbit IgG |
| Clone | 4P145 |
| Reactivity | Human |
| Specificity | Human, mouse
Species predicted to react based on 100% sequence homology: RatPhospho-ERK1/2 (Thr202, Tyr204) |
| Verified Activity | 1. Western blot analysis of extracts from serum-starved Hela, untreated (-) or treated with Calyculin A (100 nM, 30 min; +), using Phospho-p44/42 MAPK (ERK1/2) (Thr202, Tyr204) rabbit monoclonal Antibody (upper), or Anti-ERK2 rabbit polyclonal antibody at 1:1000 dilution (lower).
2. Western blot analysis of extracts from serum-starved Hela, untreated (line A); treated with Calyculin A (100 nM, 30 min), without peptide (line B) or antigen-specific phosphopeptide (line C) or antigen-specific peptide (line D) using Phospho-p44/42 MAPK (ERK1/2) (Thr202, Tyr204) rabbit monoclonal Antibody at 1:1000 dilution. (Validation Experiment) 3. Western blot analysis of 15 μg extracts from serum-starved NIH-3T3, untreated (-); treated with PDGFA (5 μg/mL, 5 min; +), using Phospho-ERK1/2 (Thr202, Tyr204) Antibody, Rabbit MAb at 1:1000 dilution. (Validation Experiment) 4. Western blot analysis of extracts from serum-starved Hela, treated with Calyculin A (100 nM, 30 min), using Phospho-p44/42 MAPK (ERK1/2) (Thr202, Tyr204) rabbit monoclonal Antibody and other brands’ antibodies (company C) at dilution of 1:1000, 1:10000 and 1:200000. (Validation Experiment) |
| Application | |
| Recommended Dose | WB: 1:1000-1:20000 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | This antibody was obtained from a rabbit immunized with a synthetic phosphopeptide corresponding to residues around Thr202, Tyr204 of Human Phospho-ERK1/2. |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 10 mM sodium HEPES, 150 mM NaCl, 100 μg/mL BSA, 50% glycerol, pH 7.5 |
| Research Background | MAPK3 (Mitogen-Activated Protein Kinase 3, also known as ERK1) is a Protein Coding gene. The protein encoded by the ERK1 gene is a member of the MAP kinase family. 3 alternatively spliced human isoforms have been reported. The protein is a serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs that play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. ERK1 is widely expressed in the small intestine, colon, and other tissues. Diseases associated with MAPK3 include Neutrophil Migration and Pertussis.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy |
| Conjucates | Unconjugated |
| Immunogen | A synthetic peptide: residues around Thr202, Tyr204 of Human Phospho-ERK1/2. |
| Antigen Species | Human |
| Biology Area | Cancer Drug Targets |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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