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Anti-Phospho-ERK1/2 (Thr183, Tyr185) Polyclonal Antibody
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Catalog No. TMAB-01408
Anti-Phospho-ERK1/2 (Thr183, Tyr185) Polyclonal Antibody is a Rabbit antibody targeting Phospho-ERK1/2 (Thr183, Tyr185). Anti-Phospho-ERK1/2 (Thr183, Tyr185) Polyclonal Antibody can be used in ICC/IF.
Anti-Phospho-ERK1/2 (Thr183, Tyr185) Polyclonal Antibody
Anti-Phospho-ERK1/2 (Thr183, Tyr185) Polyclonal Antibody
😃Good
Catalog No. TMAB-01408
Anti-Phospho-ERK1/2 (Thr183, Tyr185) Polyclonal Antibody is a Rabbit antibody targeting Phospho-ERK1/2 (Thr183, Tyr185). Anti-Phospho-ERK1/2 (Thr183, Tyr185) Polyclonal Antibody can be used in ICC/IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $221 | 7-10 days | |
| 100 μL | $374 | 7-10 days | |
| 200 μL | $527 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Antibody Type: Rabbit Polyclonal Application: ICC/IF Reactivity: Human (predicted:Mouse,Rat,Chicken,Dog,Cow,Horse,Rabbit,GuineaPig) |
| Ig Type | IgG |
| Reactivity | Human (predicted:Mouse,Rat,Chicken,Dog,Cow,Horse,Rabbit,GuineaPig) |
| Verified Activity | 1. Tissue/cell: Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (phospho-ERK1 + 2 (Thr183/Tyr185)) polyclonal Antibody, Unconjugated (TMAB-01408) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus. 2. Tissue/cell: HUVEC cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (phospho-ERK1 + 2 (Thr183/Tyr185)) Polyclonal Antibody, Unconjugated (TMAB-01408) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus. |
| Application | ICC/IF |
| Recommended Dose | ICC/IF: 1:100 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cytoplasm, cytoskeleton, spindle. Nucleus. Cytoplasm, cytoskeleton, centrosome. Cytoplasm. Note=Associated with the spindle duringprometaphase and metaphase. PEA15-binding andphosphorylated DAPK1 promote its cytoplasmic retention.Phosphorylation at Ser-244 and Ser-246 as well asautophosphorylation at Thr-188 promote nuclear localization. |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | The protein encoded by this gene is a member of the MAPkinase family. MAP kinases, also known as extracellularsignal-regulated kinases (ERKs), act in a signaling cascade thatregulates various cellular processes such as proliferation,differentiation, and cell cycle progression in response to avariety of extracellular signals. This kinase is activated byupstream kinases, resulting in its translocation to the nucleuswhere it phosphorylates nuclear targets. Alternatively splicedtranscript variants encoding different protein isoforms have beendescribed. [provided by RefSeq, Jul 2008]. |
| Immunogen | KLH conjugated Synthesised phosphopeptide: mouse ERK1 around the phosphorylation site of Thr183/Tyr185 |
| Antigen Species | Mouse |
| Biology Area | Tangles & Tau,MAPK Pathway,Cytoplasmic |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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