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Anti-Phospho-ERK1/2 (T185) Antibody (1B497)

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Catalog No. TMAC-03213

Anti-Phospho-ERK1/2 (T185) Antibody (1B497) is a Rabbit antibody targeting Phospho-ERK1/2 (T185). Anti-Phospho-ERK1/2 (T185) Antibody (1B497) can be used in WB,ICC,IHC,FCM.

Anti-Phospho-ERK1/2 (T185) Antibody (1B497)

Anti-Phospho-ERK1/2 (T185) Antibody (1B497)

😃Good
Catalog No. TMAC-03213
Anti-Phospho-ERK1/2 (T185) Antibody (1B497) is a Rabbit antibody targeting Phospho-ERK1/2 (T185). Anti-Phospho-ERK1/2 (T185) Antibody (1B497) can be used in WB,ICC,IHC,FCM.
Pack SizePriceAvailabilityQuantity
50 μL$2987-10 days
100 μL$4987-10 days
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Product Introduction

Bioactivity
Description
Anti-Phospho-ERK1/2 (T185) Antibody (1B497) is a Rabbit antibody targeting Phospho-ERK1/2 (T185). Anti-Phospho-ERK1/2 (T185) Antibody (1B497) can be used in WB,ICC,IHC,FCM.
Ig Type
IgG
Reactivity
Human
Application
Recommended Dose
WB: 1:1000; IHC: 1:50-200; ICC: 1:100-500; FCM: 1:50-100
Antibody Type
Monoclonal
Host SpeciesRabbit
ConstructionRecombinant Antibody
PurificationProA affinity purified
AppearanceLiquid
Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
Research BackgroundMitogen-activated protein kinases (MAPKs) are a widely conserved family of serine/threonine protein kinases involved in many cellular programs, such as cell proliferation, differentiation, motility, and death. The p44/42 MAPK (Erk1/2) signaling pathway can be activated in response to a diverse range of extracellular stimuli including mitogens, growth factors, and cytokines, and research investigators consider it an important target in the diagnosis and treatment of cancer. Upon stimulation, a sequential three-part protein kinase cascade is initiated, consisting of a MAP kinase kinase kinase (MAPKKK or MAP3K), a MAP kinase kinase (MAPKK or MAP2K), and a MAP kinase (MAPK). Multiple p44/42 MAP3Ks have been identified, including members of the Raf family, as well as Mos and Tpl2/COT. MEK1 and MEK2 are the primary MAPKKs in this pathway. MEK1 and MEK2 activate p44 and p42 through phosphorylation of activation loop residues Thr202/Tyr204 and Thr185/Tyr187, respectively. Several downstream targets of p44/42 have been identified, including p90RSK and the transcription factor Elk-1. p44/42 are negatively regulated by a family of dual-specificity (Thr/Tyr) MAPK phosphatases, known as DUSPs or MKPs, along with MEK inhibitors, such as U0126 and PD98059.
Related Conjugates and Formulations
Conjucates
Unconjugated
Others Formats
Phospho
Antigen Details
Immunogen
A synthesized phosphopeptide: human Erk2 around the phosphorylation site of Thr185
Antigen Species
human
Uniprot ID
Chemical Properties
Molecular WeightTheoretical: 42/44 kDa.
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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