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Anti-Phospho-ER alpha (Tyr537) Polyclonal Antibody is a Rabbit antibody targeting Phospho-ER alpha (Tyr537). Anti-Phospho-ER alpha (Tyr537) Polyclonal Antibody can be used in WB,IHC.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $217 | 7-10 days | |
100 μL | $316 | 7-10 days |
Description | Anti-Phospho-ER alpha (Tyr537) Polyclonal Antibody is a Rabbit antibody targeting Phospho-ER alpha (Tyr537). Anti-Phospho-ER alpha (Tyr537) Polyclonal Antibody can be used in WB,IHC. |
Synonyms | p-ER alpha (Y537), p-ER alpha (Tyr537), ER alpha (p-Y537), ER alpha (p-Tyr537) |
Ig Type | IgG |
Reactivity | Human,Mouse |
Verified Activity | 1. Western blot analysis of extracts from K562 cells (Lane 2), using Estrogen Receptor-α (Phospho-Tyr537) Antibody TMAC-01395. The lane on the left is treated with antigen-specific peptide. 2. Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using Estrogen Receptor-α (Phospho-Tyr537) antibody TMAC-01395 (left)or the same antibody preincubated with blocking peptide (right). |
Application | |
Recommended Dose | WB: 1:500-1000; IHC: 1:50-100 |
Antibody Type | Polyclonal |
Host Species | Rabbit |
Construction | Polyclonal Antibody |
Purification | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
Appearance | Liquid |
Formulation | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Research Background | Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. |
Conjucates | Unconjugated |
Others Formats | Phospho |
Immunogen | Peptide sequence around phosphorylation site of tyrosine 537(P-L-Y(p)-D-L) derived from Human Estrogen Receptor-α |
Antigen Species | human |
Uniprot ID |
Molecular Weight | Actual: 66 kDa. |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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