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Anti-Phospho-Ephrin B (Ser300) Polyclonal Antibody
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Catalog No. TMAB-10658
Anti-Phospho-Ephrin B (Ser300) Polyclonal Antibody is a Rabbit antibody targeting Phospho-Ephrin B (Ser300). Anti-Phospho-Ephrin B (Ser300) Polyclonal Antibody can be used in WB.
Anti-Phospho-Ephrin B (Ser300) Polyclonal Antibody
Anti-Phospho-Ephrin B (Ser300) Polyclonal Antibody
😃Good
Catalog No. TMAB-10658
Anti-Phospho-Ephrin B (Ser300) Polyclonal Antibody is a Rabbit antibody targeting Phospho-Ephrin B (Ser300). Anti-Phospho-Ephrin B (Ser300) Polyclonal Antibody can be used in WB.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $221 | 7-10 days | 7-10 days | |
| 100 μL | $371 | 7-10 days | 7-10 days | |
| 200 μL | $527 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-Phospho-Ephrin B (Ser300) Polyclonal Antibody is a Rabbit antibody targeting Phospho-Ephrin B (Ser300). Anti-Phospho-Ephrin B (Ser300) Polyclonal Antibody can be used in WB. |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat (predicted:Chicken,Dog,Pig,Cow,Horse,Rabbit) |
| Application | |
| Recommended Dose | WB: 1:500-2000 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Membrane; Single-pass type I membrane protein. |
| Tissue Specificity | Highly expressed in brain; expressed in embryonic floor plate, roof plate and hindbrain segments. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | Ephrin B proteins are thought to play key roles in cellular functions as diverse as neuronal migration and blood vessel development. Ephrin B molecules expressed at the membrane surface bind to the Ephrin B family receptors on target cells during cell to cell contact. This interaction leads to cell signaling in the target cell but also generates a reverse signal in the cell expressing Ephrin B on its surface. This reverse signaling event is thought to be critical for vessel maturation and neuronal development. Importantly, tyrosine phosphorylation of Ephrin B is thought to be a critical component of this reverse signaling event. Recent work demonstrated that Tyr331 of Ephrin B was phosphorylated in HEK293 cells after stimulation by the soluble Ephrin B2 receptor tyrosine kinase. |
| Immunogen | KLH conjugated Synthesised phosphopeptide: human Ephrin B around the phosphorylation site of Ser300 |
| Antigen Species | Human |
| Gene Name | EFNB1 |
| Gene ID | |
| Protein Name | Ephrin-B1 |
| Uniprot ID | |
| Function | Binds to the receptor tyrosine kinases EPHB1 and EPHA1. Binds to, and induce the collapse of, commissural axons/growth cones in vitro. May play a role in constraining the orientation of longitudinally projecting axons.
Cell surface transmembrane ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Binds to the receptor tyrosine kinases EPHB3 (preferred), EPHB1 and EPHA1. Binds to, and induce the collapse of, commissural axons/growth cones in vitro. May play a role in constraining the orientation of longitudinally projecting axons. |
| Molecular Weight | Theoretical: 38 kDa. |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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