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Anti-Phospho-EphA2 (Tyr588, 596) Polyclonal Antibody is a Rabbit antibody targeting Phospho-EphA2 (Tyr588, 596). Anti-Phospho-EphA2 (Tyr588, 596) Polyclonal Antibody can be used in WB,IF.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
100 μL | $317 | 7-10 days |
Description | Anti-Phospho-EphA2 (Tyr588, 596) Polyclonal Antibody is a Rabbit antibody targeting Phospho-EphA2 (Tyr588, 596). Anti-Phospho-EphA2 (Tyr588, 596) Polyclonal Antibody can be used in WB,IF. |
Synonyms | p-EphA2 (Y588, 596), p-EphA2 (Tyr588, 596), EphA2 (p-Y588, 596), EphA2 (p-Tyr588, 596) |
Ig Type | IgG |
Reactivity | Human,Mouse,Rat |
Verified Activity | 1. Western blot analysis of EPHA2 (phospho-Tyr588/596) expression in A431, mouse brain whole cell lysates. 2. Immunofluorescent analysis of EPHA2 (phospho-Tyr588/596) staining in A431 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue). |
Application | |
Recommended Dose | WB: 1:500-1000; IF:1:100-300 |
Antibody Type | Polyclonal |
Host Species | Rabbit |
Construction | Polyclonal Antibody |
Purification | The antibody was purified by immunogen affinity chromatography. |
Appearance | Liquid |
Formulation | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Conjucates | Unconjugated |
Others Formats | Phospho |
Immunogen | KLH conjugated synthetic peptide: encompassing a sequence within the center region of human EPHA2 |
Antigen Species | human |
Uniprot ID |
Molecular Weight | Theoretical: 130 kDa. |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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