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Anti-Phospho-eNOS (Thr495) Polyclonal Antibody is a Rabbit antibody targeting Phospho-eNOS (Thr495). Anti-Phospho-eNOS (Thr495) Polyclonal Antibody can be used in WB.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $218 | 7-10 days | 7-10 days | |
| 100 μL | $317 | 7-10 days | 7-10 days |
| Description | Anti-Phospho-eNOS (Thr495) Polyclonal Antibody is a Rabbit antibody targeting Phospho-eNOS (Thr495). Anti-Phospho-eNOS (Thr495) Polyclonal Antibody can be used in WB. |
| Synonyms | p-eNOS (Thr495), p-eNOS (T495), eNOS (p-Thr495), eNOS (p-T495) |
| Ig Type | IgG |
| Reactivity | Human |
| Verified Activity | 1. Western blot analysis of extracts from Jurkat cells and HepG2 cells using eNOS (Phospho-Thr495) Antibody TMAC-01299. The lane on the right is treated with the antigen-specific peptide. ![]() |
| Application | |
| Recommended Dose | WB: 1:500-1000 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Construction | Polyclonal Antibody |
| Purification | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| Appearance | Liquid |
| Formulation | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Research Background | Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets. |
| Conjucates | Unconjugated |
| Others Formats | Phospho |
| Immunogen | Peptide sequence around phosphorylation site of threonine 495 (K-K-T(p)-F-K derived from Human eNOS |
| Antigen Species | human |
| Uniprot ID |
| Molecular Weight | Actual: 140 kDa. |
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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