Anti-Phospho-eNOS (Thr113) Polyclonal Antibody is a Rabbit antibody targeting Phospho-eNOS (Thr113). Anti-Phospho-eNOS (Thr113) Polyclonal Antibody can be used in FCM.

IgG

Human (predicted:Mouse,Rat,Dog,Rabbit)

Blank control: U937.
Primary Antibody (green line): Rabbit Anti-Phospho-eNOS (Thr113) antibody (TMAB-10648)
Dilution: 2 μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-PE
Dilution: 1 μg /test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

FCM: 2μg/Test

Polyclonal

KLH conjugated Synthesised phosphopeptide: mouse eNOS around the phosphorylation site of Thr113

Mouse

NOS3

Nitric oxide synthase, endothelial

Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.
Isoform eNOS13C: Lacks eNOS activity, dominant-negative form that may down-regulate eNOS activity by forming heterodimers with isoform 1.