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Anti-Phospho-eNOS (Ser1177) Polyclonal Antibody
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Catalog No. TMAB-01405
Anti-Phospho-eNOS (Ser1177) Polyclonal Antibody is a Rabbit antibody targeting Phospho-eNOS (Ser1177). Anti-Phospho-eNOS (Ser1177) Polyclonal Antibody can be used in WB.
Anti-Phospho-eNOS (Ser1177) Polyclonal Antibody
Anti-Phospho-eNOS (Ser1177) Polyclonal Antibody
😃Good
Catalog No. TMAB-01405
Anti-Phospho-eNOS (Ser1177) Polyclonal Antibody is a Rabbit antibody targeting Phospho-eNOS (Ser1177). Anti-Phospho-eNOS (Ser1177) Polyclonal Antibody can be used in WB.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $220 | 7-10 days | |
| 100 μL | $372 | 7-10 days | |
| 200 μL | $529 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Antibody Type: Rabbit Polyclonal Application: WB Reactivity: Human (predicted:Mouse,Rat) |
| Ig Type | IgG |
| Reactivity | Human (predicted:Mouse,Rat) |
| Verified Activity | Sample: Lane 1: Human HUVEC cell lysates Lane 2: Human HUVEC treated with 100 µM Pervanadate (PV) for 10 minutes Primary: Anti-Phospho-eNOS (Ser1177) (TMAB-01405) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 133 kDa Observed band size: 135 kDa |
| Application | WB |
| Recommended Dose | WB: 1:500-1000 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cell membrane. Membrane, caveola. Cytoplasm, cytoskeleton. Golgi apparatus. Note=Specifically associates with actin cytoskeleton in the G2 phase of the cell cycle; which is favored by interaction with NOSIP and results in a reduced enzymatic activity. |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | Nitric oxide synthase NOS oxidizes a guanidine nitrogen of arginine releasing nitric oxide in the form of a free radical and citrulline. Nitric oxide thus generated acts as a messenger in diverse functions including vasodilation neurotransmission, anti tumor and anti pathogenic activities. NOS is classified under three types: neuronal NOS (nNOS) or brain NOS (bNOS); inducible NOS (iNOS) or macrophage NOS (mNOS); and endothelial NOS (eNOS). |
| Immunogen | KLH conjugated Synthesised phosphopeptide: mouse eNOS around the phosphorylation site of Ser1177 |
| Antigen Species | Mouse |
| Gene ID | 18127 |
| Uniprot ID | |
| Biology Area | Response to hypoxia,Platelets,Nitric oxide associated,Oxidative Stress,Hypoxia,Oxidative stress,NOS |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
Sci Citations
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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