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Anti-Phospho-eNOS (Ser1177) Polyclonal Antibody 2
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Catalog No. TMAC-01302
Alias p-eNOS (Ser1177), p-eNOS (S1177), eNOS (p-Ser1177), eNOS (p-S1177)
Anti-Phospho-eNOS (Ser1177) Polyclonal Antibody is a Rabbit antibody targeting Phospho-eNOS (Ser1177). Anti-Phospho-eNOS (Ser1177) Polyclonal Antibody can be used in WB,IF.
Anti-Phospho-eNOS (Ser1177) Polyclonal Antibody 2
Anti-Phospho-eNOS (Ser1177) Polyclonal Antibody 2
😃Good
Catalog No. TMAC-01302Alias p-eNOS (Ser1177), p-eNOS (S1177), eNOS (p-Ser1177), eNOS (p-S1177)
Anti-Phospho-eNOS (Ser1177) Polyclonal Antibody is a Rabbit antibody targeting Phospho-eNOS (Ser1177). Anti-Phospho-eNOS (Ser1177) Polyclonal Antibody can be used in WB,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $218 | 7-10 days | |
| 100 μL | $317 | 7-10 days |
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Product Introduction
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Antigen Details
Chemical Properties
| Description | Anti-Phospho-eNOS (Ser1177) Polyclonal Antibody is a Rabbit antibody targeting Phospho-eNOS (Ser1177). Anti-Phospho-eNOS (Ser1177) Polyclonal Antibody can be used in WB,IF. |
| Synonyms | p-eNOS (Ser1177), p-eNOS (S1177), eNOS (p-Ser1177), eNOS (p-S1177) |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Western Blot analysis of HuvEc cells using Phospho-NOS3 (S1177) Polyclonal Antibody diluted at 1:1000. 2. Western blot analysis of lysates from HeLa cells treated with Insulin 0.01U/ml 15', using eNOS (Phospho-Ser1176) Antibody. The lane on the right is blocked with the phospho peptide. 3. Immunofluorescence analysis of A549. 1,primary Antibody(red) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit IgG (H&L) - Alexa Fluor 594 Secondary antibody was diluted at 1:1000 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. 4. Immunofluorescence analysis of Hela cell. 1,NOS3 (phospho Ser1177) Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 was diluted at 1:1000 (room temperature, 50min). 3 DAPI (blue) 10min. |
| Application | |
| Recommended Dose | WB: 1:500-2000; IF: 1:200-1000 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Construction | Polyclonal Antibody |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Appearance | Liquid |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research Background | Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009], |
| Conjucates | Unconjugated |
| Others Formats | Phospho |
| Immunogen | A synthesized phosphopeptide: human eNOS around the phosphorylation site of Ser1176 |
| Antigen Species | human |
| Uniprot ID |
| Molecular Weight | Theoretical: 130-140 kDa. |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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