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Anti-Phospho-eNOS (Ser1177) Antibody (4B383)
Catalog No. TMAB-10646 Copy Product Info
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Anti-Phospho-eNOS (Ser1177) Antibody (4B383) is a Rabbit antibody targeting Phospho-eNOS (Ser1177). Anti-Phospho-eNOS (Ser1177) Antibody (4B383) can be used in IHC-P,IHC-Fr,IF.
Anti-Phospho-eNOS (Ser1177) Antibody (4B383)
Copy Product Info🥰Excellent
Catalog No. TMAB-10646
Anti-Phospho-eNOS (Ser1177) Antibody (4B383) is a Rabbit antibody targeting Phospho-eNOS (Ser1177). Anti-Phospho-eNOS (Ser1177) Antibody (4B383) can be used in IHC-P,IHC-Fr,IF.
Anti-Phospho-eNOS
(Ser1177) Antibody
(4B383)
(Ser1177) Antibody
(4B383)
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 25 μL | $150 | 7-10 days | 7-10 days | |
| 50 μL | $264 | 7-10 days | 7-10 days | |
| 100 μL | $473 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction
Bioactivity
Antigen Details
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| Description | Anti-Phospho-eNOS (Ser1177) Antibody (4B383) is a Rabbit antibody targeting Phospho-eNOS (Ser1177). Anti-Phospho-eNOS (Ser1177) Antibody (4B383) can be used in IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Clone | 4B383 |
| Reactivity | Human |
| Verified Activity | Paraformaldehyde-fixed, paraffin embedded (human placenta); Antigen retrieval by boiling in EDTA buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (Phospho-eNOS (Ser1177)) Monoclonal Antibody, Unconjugated (TMAB-10646) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. |
| Application | |
| Recommended Dose | IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cell membrane. Membrane, caveola. Cytoplasm, cytoskeleton. Golgi apparatus. Note=Specifically associates with actin cytoskeleton in the G2 phase of the cell cycle; which is favored by interaction with NOSIP and results in a reduced enzymatic activity. |
| Tissue Specificity | Platelets, placenta, liver and kidney. |
| Construction | Recombinant Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | Nitric oxide synthase NOS oxidizes a guanidine nitrogen of arginine releasing nitric oxide in the form of a free radical and citrulline. Nitric oxide thus generated acts as a messenger in diverse functions including vasodilation neurotransmission, anti tumor and anti pathogenic activities. NOS is classified under three types: neuronal NOS (nNOS) or brain NOS (bNOS); inducible NOS (iNOS) or macrophage NOS (mNOS); and endothelial NOS (eNOS). |
| Immunogen | KLH conjugated Synthesised phosphopeptide: human eNOS around the phosphorylation site of Ser1177: |
| Antigen Species | Human |
| Gene Name | NOS3 |
| Gene ID | |
| Protein Name | Nitric oxide synthase, endothelial |
| Uniprot ID | |
| Function | Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.
Isoform eNOS13C: Lacks eNOS activity, dominant-negative form that may down-regulate eNOS activity by forming heterodimers with isoform 1. |
| Molecular Weight | Theoretical: 133 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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