Anti-Phospho-ELk1 (Ser389) Polyclonal Antibody 2 is a Rabbit antibody targeting Phospho-ELk1 (Ser389). Anti-Phospho-ELk1 (Ser389) Polyclonal Antibody 2 can be used in WB,FCM.

IgG

Human,Mouse,Rat (predicted:Dog,Pig,Cow,Rabbit,Sheep,GuineaPig)

1. Sample:
Lane 1: Mouse Cerebrum tissue lysates
Lane 2: Rat Cerebrum tissue lysates
Primary: Anti-Phospho-ELk1 (Ser389) (TMAB-10644) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 47 kDa
Observed band size: 59 kDa
2. Blank control (black line): U-87MG.
Primary Antibody (green line): Rabbit Anti-Phospho-ELk1 (Ser389) antibody (TMAB-10644)
Dilution: 1 μg/Test;
Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF488
Dilution: 0.5 μg/Test.
Isotype control (orange line): Normal Rabbit IgG
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

WB: 1:500-2000; FCM: 1μg/Test

Polyclonal

KLH conjugated Synthesised phosphopeptide: human ELk1 around the phosphorylation site of Ser389

Human

ELK1

ETS domain-containing protein Elk-1

Stimulates transcription. Binds to purine-rich DNA sequences. Can form a ternary complex with the serum response factor and the ETS and SRF motifs of the fos serum response element.