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Anti-Phospho-eIF4EBP1 (Thr70) Polyclonal Antibody
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Catalog No. TMAB-10640
Anti-Phospho-eIF4EBP1 (Thr70) Polyclonal Antibody is a Rabbit antibody targeting Phospho-eIF4EBP1 (Thr70). Anti-Phospho-eIF4EBP1 (Thr70) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-Phospho-eIF4EBP1 (Thr70) Polyclonal Antibody
Anti-Phospho-eIF4EBP1 (Thr70) Polyclonal Antibody
Copy Product Info😃Good
Catalog No. TMAB-10640
Anti-Phospho-eIF4EBP1 (Thr70) Polyclonal Antibody is a Rabbit antibody targeting Phospho-eIF4EBP1 (Thr70). Anti-Phospho-eIF4EBP1 (Thr70) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $222 | 7-10 days | 7-10 days | |
| 100 μL | $373 | 7-10 days | 7-10 days | |
| 200 μL | $527 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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| Description | Anti-Phospho-eIF4EBP1 (Thr70) Polyclonal Antibody is a Rabbit antibody targeting Phospho-eIF4EBP1 (Thr70). Anti-Phospho-eIF4EBP1 (Thr70) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Rat (predicted:Human,Mouse,Chicken,Dog,Cow,Horse,Sheep) |
| Verified Activity | 1. Sample: Ovary (Rat) Tissue Lysate at 30 μg Primary: Anti-phospho-eIF4EBP1 (Thr70) (TMAB-10640) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 12 kD Observed band size: 12 kD 2. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0); Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30 min; Antibody incubation with (phospho-eIF4EBP1 (Thr70)) Polyclonal Antibody, Unconjugated (TMAB-10640) at 1:400 overnight at 4°C, followed by conjugation to the secondary antibody (labeled with HRP) and DAB staining. |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Nucleus. |
| Tissue Specificity | Lung and testis. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation. [provided by RefSeq, Jul 2008]. |
| Immunogen | KLH conjugated Synthesised phosphopeptide: human EIF4B around the phosphorylation site of Thr70 |
| Antigen Species | Human |
| Gene Name | EIF4EBP1 |
| Gene ID | |
| Protein Name | Eukaryotic translation initiation factor 4E-binding protein 1 |
| Uniprot ID | |
| Function | Regulates eIF4E activity by preventing its assembly into the eIF4F complex. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways. |
| Molecular Weight | Theoretical: 12 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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