Anti-Phospho-eIF4EBP1/2/3(Thr46/Thr46/Thr32) Antibody (5K7) is a Rabbit antibody targeting Phospho-eIF4EBP1/2/3(Thr46/Thr46/Thr32). Anti-Phospho-eIF4EBP1/2/3(Thr46/Thr46/Thr32) Antibody (5K7) can be used in WB,IHC-P,IHC-Fr,IF.

IgG

5K7

Human,Mouse

1. Western blot analysis of 293 treated with FBS+293. Using Phospho-eIF4EBP1/2/3 (Thr46/Thr46/Thr32) (TMAB-10641) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
2. Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Phospho-eIF4EBP1/2/3 (Thr46/Thr46/Thr32) Monoclonal Antibody, Unconjugated (TMAB-10641) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Phospho-eIF4EBP1/2/3 (Thr46/Thr46/Thr32) Monoclonal Antibody, Unconjugated (TMAB-10641) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Phospho-eIF4EBP1/2/3 (Thr46/Thr46/Thr32) Monoclonal Antibody, Unconjugated (TMAB-10641) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded Human Pancreatic Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Phospho-eIF4EBP1/2/3 (Thr46/Thr46/Thr32) Monoclonal Antibody, Unconjugated (TMAB-10641) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.

WB: 1:500-2000; IHC-P: 1:50-200; IHC-Fr: 1:50-200; IF: 1:50-200

Monoclonal

A synthesized peptide: human 4E BP1 around the phosphorylation site of T46

Human

EIF4EBP1

Eukaryotic translation initiation factor 4E-binding protein 1

Regulates eIF4E activity by preventing its assembly into the eIF4F complex. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways.