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Anti-Phospho-eIF4E (Ser209) Antibody (6B314)
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Catalog No. TMAB-10634
Anti-Phospho-eIF4E (Ser209) Antibody (6B314) is a Rabbit antibody targeting Phospho-eIF4E (Ser209). Anti-Phospho-eIF4E (Ser209) Antibody (6B314) can be used in WB,IHC-P,IHC-Fr,IF.
Anti-Phospho-eIF4E (Ser209) Antibody (6B314)
Anti-Phospho-eIF4E (Ser209) Antibody (6B314)
😃Good
Catalog No. TMAB-10634
Anti-Phospho-eIF4E (Ser209) Antibody (6B314) is a Rabbit antibody targeting Phospho-eIF4E (Ser209). Anti-Phospho-eIF4E (Ser209) Antibody (6B314) can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $261 | 7-10 days | 7-10 days | |
| 100 μL | $470 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-Phospho-eIF4E (Ser209) Antibody (6B314) is a Rabbit antibody targeting Phospho-eIF4E (Ser209). Anti-Phospho-eIF4E (Ser209) Antibody (6B314) can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Clone | 6B314 |
| Reactivity | Human,Mouse,Rat (predicted:Chicken,Dog,Pig,Cow,Horse,Rabbit) |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:50-200; IHC-Fr: 1:50-200; IF: 1:50-200 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cytoplasm, P-body. |
| Construction | Recombinant Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | eIF4E, a protein modulates translation of maternal mRNAs in early embryos before the onset of zygotic transcription. eIF4E also influences the overall rate of translation. eIF4E binds to the 7 methyl GTP cap structure of eukaryotic mRNAs. Phosphorylation of eIF4E on serine 209 regulates the affinity of this protein for the 7 methyl GTP cap and/or RNA. Phosphorylation also enhances the interaction of eIF4E with eIF4G, which form a complex known as eIF4F. eIF4E phosphorylation is correlated with increased translational rate in a number of cell types. Several kinases are currently being investigated as potential regulators of eIF4E including PKC and/or the MAP kinase activated Mnk. |
| Immunogen | KLH conjugated Synthesised phosphopeptide: human eIF4E around the phosphorylation site of Ser209 |
| Antigen Species | Human |
| Gene Name | EIF4E |
| Gene ID | |
| Protein Name | Eukaryotic translation initiation factor 4E |
| Uniprot ID | |
| Function | Its translation stimulation activity is repressed by binding to the complex CYFIP1-FMR1 (By similarity). Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap. |
| Molecular Weight | Theoretical: 24 kDa. |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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