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Anti-Phospho-eIF2α (Ser51) Antibody (1F908) (Synonyms: Phospho-eIF2α (S51), p-eIF2α (Ser51), p-eIF2α (S51), e...)
Catalog No. TMAC-03210 Copy Product Info
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Anti-Phospho-eIF2α (Ser51) Antibody (1F908) is a Rabbit antibody targeting Phospho-eIF2α (Ser51). Anti-Phospho-eIF2α (Ser51) Antibody (1F908) can be used in FCM,ICC/IF,IHC,IP,WB.
Anti-Phospho-eIF2α (Ser51) Antibody (1F908)
Copy Product Info🥰Excellent
Catalog No. TMAC-03210
Synonyms Phospho-eIF2α (S51), p-eIF2α (Ser51), p-eIF2α (S51), eIF2α (p-Ser51), eIF2α (p-S51), EIF-2α, EIF-2alpha, EIF2alpha, EIF-2A, EIF2A, EIF-2, EIF2
Anti-Phospho-eIF2α (Ser51) Antibody (1F908) is a Rabbit antibody targeting Phospho-eIF2α (Ser51). Anti-Phospho-eIF2α (Ser51) Antibody (1F908) can be used in FCM,ICC/IF,IHC,IP,WB.
Anti-Phospho-eIF2α
(Ser51) Antibody
(1F908)
(Ser51) Antibody
(1F908)
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 50 μL | $298 | 7-10 days | 7-10 days | |
| 100 μL | $496 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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| Description | Anti-Phospho-eIF2α (Ser51) Antibody (1F908) is a Rabbit antibody targeting Phospho-eIF2α (Ser51). Anti-Phospho-eIF2α (Ser51) Antibody (1F908) can be used in FCM,ICC/IF,IHC,IP,WB. |
| Synonyms | Phospho-eIF2α (S51), p-eIF2α (Ser51), p-eIF2α (S51), eIF2α (p-Ser51), eIF2α (p-S51), EIF-2α, EIF-2alpha, EIF2alpha, EIF-2A, EIF2A, EIF-2, EIF2 |
| Ig Type | IgG |
| Clone | 1F908 |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Western blot analysis of Phospho-eIF-2a (S51) on different lysates using anti-Phospho-eIF-2a (S51) antibody at 1/1,000 dilution. Positive control: Lane 1: Hela, Lane 2: HUVEC, Lane 3: PC12. 2. Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Phospho-eIF-2a (S51) antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-Phospho-eIF-2a (S51) antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Phospho-eIF-2a (S51) antibody. Counter stained with hematoxylin. 5. Immunohistochemical analysis of paraffin-embedded mouse placenta tissue using anti-Phospho-eIF-2a (S51) antibody. Counter stained with hematoxylin. 6. Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-Phospho-eIF-2a (S51) antibody. Counter stained with hematoxylin. 7. ICC staining Phospho-eIF-2a (S51) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 8. Flow cytometric analysis of Hela cells with Phospho-eIF-2a (S51) antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.         |
| Application | |
| Recommended Dose | WB: 1:1000; IHC: 1:50-200; ICC/IF: 1:50-200; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | Phosphorylation of the eukaryotic initiation factor 2 (eIF2) α subunit is a well-documented mechanism to downregulate protein synthesis under a variety of stress conditions. Eukaryotic initiation factor 2 binds GTP and Met-tRNAi and transfers Met-tRNA to the 40S subunit to form the 43S preinitiation complex. eIF2 promotes a new round of translation initiation by exchanging GDP for GTP, a reaction catalyzed by eIF2B. Kinases that are activated by viral infection (PKR), endoplasmic reticulum stress (PERK/PEK), amino acid deprivation (GCN2), or heme deficiency (HRI) can phosphorylate the α subunit of eIF2. This phosphorylation stabilizes the eIF2-GDP-eIF2B complex and inhibits the turnover of eIF2B. Induction of PKR by IFN-γ and TNF-α induces potent phosphorylation of eIF2α at Ser51. |
| Conjucates | Unconjugated |
| Others Formats | Phospho |
| Immunogen | A synthesized phosphopeptide: human eIF-2a around the phosphorylation site of Ser51 |
| Antigen Species | human |
| Uniprot ID |
| Molecular Weight | Theoretical: 36 kDa. |
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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