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Anti-Phospho-eEF2 (Thr56) Antibody (6P325) is a Rabbit antibody targeting Phospho-eEF2 (Thr56). Anti-Phospho-eEF2 (Thr56) Antibody (6P325) can be used in WB.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 20 μL | $81 | 7-10 days | 7-10 days | |
| 100 μL | $235 | 7-10 days | 7-10 days |
| Description | Anti-Phospho-eEF2 (Thr56) Antibody (6P325) is a Rabbit antibody targeting Phospho-eEF2 (Thr56). Anti-Phospho-eEF2 (Thr56) Antibody (6P325) can be used in WB. |
| Synonyms | SCA26, Phospho-eEF2 (T56), p-eEF2 (Thr56), p-eEF2 (T56), EF-2, EF2, eEF2 (pThr56), eEF2 (pT56), EEF-2, EEF2 |
| Ig Type | Rabbit IgG |
| Clone | 6P325 |
| Reactivity | Human;
Predicted to React with: HumanSpecies predicted to react based on 100% sequence homology: Mouse, Rat, Bovine |
| Specificity | Human Phospho-eEF2 (Thr56), which correspond to Phospho-eEF2 (Thr57) of P13639 in UniProt.
Reacts with: Human Species predicted to react based on 100% sequence homology: Mouse, Rat, Bovine |
| Verified Activity | 1. Western blot analysis of extracts from serum-starved HepG2 , untreated(line A) or treated with forskolin(30μM, 30min)(line B), using Phospho-eEF2 (Thr56) rabbit monoclonal Antibody at 1:10000 dilution (upper) or Anti-Actin Antibody, Chimeric Rabbit Monoclonal at 1:50000 dilution (lower).
2. Western blot analysis of extracts from serum-starved HepG2 , untreated (line A); treated with forskolin(30μM, 30min), without peptide (line B) or antigen-specific phosphopeptide (line C) or antigen-specific peptide (line D) using Phospho-eEF2 (Thr56) rabbit monoclonal Antibody at 1:10000 dilution. (Validation Experiment) 3. Western blot analysis of extracts from serum-starved HepG2, untreated (line A); treated with forskolin(30μM, 30min; +) (line B); treated with forskolin and λ-phosphatase (line C) using Phospho-eEF2 (Thr56) rabbit monoclonal Antibody at 1:10000 dilution. (Validation Experiment) |
| Application | |
| Recommended Dose | WB: 1:10000-1:100000 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | This antibody was obtained from a rabbit immunized with a synthetic peptide corresponding to the residues around (Thr56) of Human Phospho-eEF2. |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 10 mM sodium HEPES, 150 mM NaCl, 100 μg/mL BSA, 50% glycerol, pH 7.5 |
| Conjucates | Unconjugated |
| Immunogen | A synthetic peptide: residues around (Thr56) of Human Phospho-eEF2 |
| Antigen Species | Human |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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