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Anti-Phospho-E2A (Thr355) Polyclonal Antibody is a Rabbit antibody targeting Phospho-E2A (Thr355). Anti-Phospho-E2A (Thr355) Polyclonal Antibody can be used in IHC.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $216 | 7-10 days | |
100 μL | $317 | 7-10 days |
Description | Anti-Phospho-E2A (Thr355) Polyclonal Antibody is a Rabbit antibody targeting Phospho-E2A (Thr355). Anti-Phospho-E2A (Thr355) Polyclonal Antibody can be used in IHC. |
Ig Type | IgG |
Reactivity | Human,Mouse,Rat |
Application | |
Recommended Dose | IHC: 1:50-100 |
Antibody Type | Polyclonal |
Host Species | Rabbit |
Construction | Polyclonal Antibody |
Purification | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
Appearance | Liquid |
Formulation | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Research Background | E2A is a transcription factor that plays major roles in determining tissue-specific cell fate during embryogenesis, like muscle or early B-cell differentiation. Heterodimers between E2A and tissue-specific basic helix-loop-helix (bHLH) Dimers bind DNA on E-box motifs: 5'- CANNTG-3'. Binds to the kappa-E2 site in the kappa immunoglobulin gene enhancer. Deletions in E2A have been observed in a subset of pre-B-cell acute lymphoblastic leukemia (B-ALL) cases. Two alternatively spliced human isoforms have been described. |
Conjucates | Unconjugated |
Others Formats | Phospho |
Immunogen | Peptide sequence around phosphorylation site of threonine 355 (P-S-T(p)-P-V) derived from Human E2A |
Antigen Species | human |
Uniprot ID |
Molecular Weight | Actual: 67 kDa. |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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