Anti-Phospho-Dnmt3a (Ser390+Ser393) Polyclonal Antibody is a Rabbit antibody targeting Phospho-Dnmt3a (Ser390+Ser393). Anti-Phospho-Dnmt3a (Ser390+Ser393) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human

1. Sample:
Lane 1: 293T (Human) Cell Lysate at 30 μg
Lane 2: SH-SY5Y (Human) Cell Lysate at 30 μg
Lane 3: Molt-4 (Human) Cell Lysate at 30 μg
Lane 4: HepG2 (Human) Cell Lysate at 30 μg
Primary: Anti-phospho-Dnmt3a (Ser390+Ser393) (TMAB-10605) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 120 kD
Observed band size: 120 kD
2. Paraformaldehyde-fixed, paraffin embedded (human gastric); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (phospho-Dnmt3a (Ser390+Ser393)) Polyclonal Antibody, Unconjugated (TMAB-10605) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated Synthesised phosphopeptide: human Dnmt3a around the phosphorylation site of Ser390 + Ser393

Human

DNMT3A

DNA(cytosine-5)-methyltransferase 3A

Required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. It modifies DNA in a non-processive manner and also methylates non-CpG sites. May preferentially methylate DNA linker between 2 nucleosomal cores and is inhibited by histone H1. Plays a role in paternal and maternal imprinting. Required for methylation of most imprinted loci in germ cells. Acts as a transcriptional corepressor for ZNF238. Can actively repress transcription through the recruitment of HDAC activity.