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Anti-Phospho-Dishevelled 2 (Thr224) Polyclonal Antibody

Catalog No. TMAB-10598 Copy Product Info
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Anti-Phospho-Dishevelled 2 (Thr224) Polyclonal Antibody is a Rabbit antibody targeting Phospho-Dishevelled 2 (Thr224). Anti-Phospho-Dishevelled 2 (Thr224) Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.

Anti-Phospho-Dishevelled 2 (Thr224) Polyclonal Antibody

Copy Product Info
🥰Excellent
Catalog No. TMAB-10598

Anti-Phospho-Dishevelled 2 (Thr224) Polyclonal Antibody is a Rabbit antibody targeting Phospho-Dishevelled 2 (Thr224). Anti-Phospho-Dishevelled 2 (Thr224) Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.

Anti-Phospho-Dishevelled 2
(Thr224) Polyclonal Antibody
Pack SizePriceUSA StockGlobal StockQuantity
50 μL$2237-10 days7-10 days
100 μL$3747-10 days7-10 days
200 μL$5297-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-Phospho-Dishevelled 2 (Thr224) Polyclonal Antibody is a Rabbit antibody targeting Phospho-Dishevelled 2 (Thr224). Anti-Phospho-Dishevelled 2 (Thr224) Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
Ig Type
IgG
Reactivity
Human (predicted:Mouse,Rat,Chicken,Dog,Pig,Cow,Horse,Sheep)
Verified Activity
Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (phospho-Dishevelled 2 (Thr224)) Polyclonal Antibody, Unconjugated (TMAB-10598) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
Application
Recommended Dose
IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationCell membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm, cytosol. Cytoplasmic vesicle. Note=Localizes at the cell membrane upon interaction with frizzled family members and promotes their internalization. Localizes to cytoplasmic puncta.
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundThis gene encodes a member of the dishevelled (dsh) protein family. The vertebrate dsh proteins have approximately 40% amino acid sequence similarity with Drosophila dsh. This gene encodes a 90-kD protein that undergoes posttranslational phosphorylation to form a 95-kD cytoplasmic protein, which may play a role in the signal transduction pathway mediated by multiple Wnt proteins. The mechanisms of dishevelled function in Wnt signaling are likely to be conserved among metazoans. [provided by RefSeq, Jul 2008].
Antigen Details
Immunogen
KLH conjugated synthesised phosphopeptide: human DVL2 around the phosphorylation site of Thr224
Antigen Species
Human
Gene Name
DVL2
Gene ID
Protein Name
Segment polarity protein dishevelled homolog DVL-2
Uniprot ID
Function
Participates in Wnt signaling by binding to the cytoplasmic C-terminus of frizzled family members and transducing the Wnt signal to down-stream effectors. Promotes internalization and degradation of frizzled proteins upon Wnt signaling. Plays a role both in canonical and non-canonical Wnt signaling. Plays a role in the signal transduction pathways mediated by multiple Wnt genes.
Chemical Properties
Molecular WeightTheoretical: 79 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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