Anti-Phospho-DDX58 (Thr170) Polyclonal Antibody is a Rabbit antibody targeting Phospho-DDX58 (Thr170). Anti-Phospho-DDX58 (Thr170) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Mouse (predicted:Dog,Pig,Cow,Horse,Rabbit,Sheep)

1. Paraformaldehyde-fixed, paraffin embedded (human cervical carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (phospho-DDX58 (Thr170)) Polyclonal Antibody, Unconjugated (TMAB-10588) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (phospho-DDX58 (Thr170)) Polyclonal Antibody, Unconjugated (TMAB-10588) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
3. Sample:
Lane 1: Mouse NIH/3T3 cell lysates
Lane 2: Human Jurkat cell lysates
Lane 3: Human MOLT4 cell lysates
Lane 4: Human MCF-7 cell lysates
Primary: Anti-phospho-DDX58 (Thr170) (TMAB-10588) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 106 kDa
Observed band size: 110 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated Synthesised phosphopeptide: human DDX58 around the phosphorylation site of Thr170

Human

DDX58

Antiviral innate immune response receptor RIG-I

Involved in innate immune defense against viruses. Upon interaction with intracellular dsRNA produced during viral replication, triggers a transduction cascade involving MAVS/IPS1, which results in the activation of NF-kappa-B, IRF3 and IRF7 and the induction of the expression of antiviral cytokines such as IFN-beta and RANTES (CCL5). Detects dsRNA produced from non-self dsDNA by RNA polymerase III, such as Epstein-Barr virus-encoded RNAs (EBERs). Essential for the production of interferons in response to RNA viruses including paramyxoviruses, influenza viruses, Japanese encephalitis virus and HCV.