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Anti-Phospho-DARPP32 (Thr75) Polyclonal Antibody

Catalog No. TMAB-10578 Copy Product Info
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Anti-Phospho-DARPP32 (Thr75) Polyclonal Antibody is a Rabbit antibody targeting Phospho-DARPP32 (Thr75). Anti-Phospho-DARPP32 (Thr75) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-Phospho-DARPP32 (Thr75) Polyclonal Antibody

Copy Product Info
🥰Excellent
Catalog No. TMAB-10578

Anti-Phospho-DARPP32 (Thr75) Polyclonal Antibody is a Rabbit antibody targeting Phospho-DARPP32 (Thr75). Anti-Phospho-DARPP32 (Thr75) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-Phospho-DARPP32
(Thr75) Polyclonal Antibody
Pack SizePriceUSA StockGlobal StockQuantity
50 μL$2227-10 days7-10 days
100 μL$3747-10 days7-10 days
200 μL$5277-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Instruction Manual of Detection Antibody

Product Introduction

Bioactivity
Description
Anti-Phospho-DARPP32 (Thr75) Polyclonal Antibody is a Rabbit antibody targeting Phospho-DARPP32 (Thr75). Anti-Phospho-DARPP32 (Thr75) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
Reactivity
Mouse (predicted:Human,Rat,Dog,Pig,Cow,Horse,Rabbit,GuineaPig)
Verified Activity
1. Sample:
Cerebral cortex (Mouse) Lysate at 40 μg
Primary: Anti-Phospho-DARPP32 (Thr75) (TMAB-10578) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 32 kD
Observed band size: 32 kD
2. Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Phospho-DARPP32 (Thr75)) Polyclonal Antibody, Unconjugated (TMAB-10578) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
Application
WB
IHC-P
IHC-Fr
IF
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationCytoplasm.
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundThis gene encodes a bifunctional signal transduction molecule. Dopaminergic and glutamatergic receptor stimulation regulates its phosphorylation and function as a kinase or phosphatase inhibitor. As a target for dopamine, this gene may serve as a therapeutic target for neurologic and psychiatric disorders. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011].
Antigen Details
Immunogen
KLH conjugated Synthesised phosphopeptide: human DARPP32 around the phosphorylation site of Thr75
Antigen Species
Human
Gene Name
PPP1R1B
Gene ID
84152
Protein Name
Protein phosphatase 1 regulatory subunit 1B
Uniprot ID
Q9UD71
Function
Inhibitor of protein-phosphatase 1.
Chemical Properties
Molecular WeightTheoretical: 32 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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