Anti-Phospho-Dab1 (Tyr198) Polyclonal Antibody is a Rabbit antibody targeting Phospho-Dab1 (Tyr198). Anti-Phospho-Dab1 (Tyr198) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Rat (predicted:Mouse,Chicken,Dog,Pig,Cow,Horse,Rabbit)

1. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-Phospho-Dab1 (Tyr198) Polyclonal Antibody, Unconjugated (TMAB-10573) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
2. Sample:
Lane 1: Human HeLa cell lysates
Lane 2: Human HepG2 cell lysates
Lane 3: Human MCF-7 cell lysates
Lane 4: Human SH-SY5Y cell lysates
Primary: Anti-Phospho-Dab1 (Tyr198) (TMAB-10573) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 65 kDa
Observed band size: 68 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthesised phosphopeptide: human Dab1 around the phosphorylation site of Tyr198

Human

DAB1

Disabled homolog 1

Adapter molecule functioning in neural development. May regulate SIAH1 activity.