Anti-Phospho-Cyclin H (Thr315) Polyclonal Antibody 2 is a Rabbit antibody targeting Phospho-Cyclin H (Thr315). Anti-Phospho-Cyclin H (Thr315) Polyclonal Antibody 2 can be used in IHC-P,IHC-Fr,IF.

IgG

Human (predicted:Mouse,Rat,Dog,Cow,Horse,Rabbit,Sheep)

Tissue/cell: human laryngocarcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-phospho-Cyclin H (Thr315) Polyclonal Antibody, Unconjugated (TMAB-10565) 1:400, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining

IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated Synthesised phosphopeptide: human Cyclin H around the phosphorylation site of Thr315

Human

CCNH

Cyclin-H

Regulates CDK7, the catalytic subunit of the CDK-activating kinase (CAK) enzymatic complex. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminus domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Involved in cell cycle control and in RNA transcription by RNA polymerase II. Its expression and activity are constant throughout the cell cycle.