Anti-Phospho-Cyclin B1 (Ser133) Polyclonal Antibody is a Rabbit antibody targeting Phospho-Cyclin B1 (Ser133). Anti-Phospho-Cyclin B1 (Ser133) Polyclonal Antibody can be used in ICC/IF,FCM.

IgG

Human (predicted:Mouse,Rat,Pig)

1. Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (Phospho-Cyclin B1 (Ser133)) polyclonal Antibody, Unconjugated (TMAB-10559) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.
2. Blank control (blue line): A549 (blue).
Primary Antibody (green line): Rabbit Anti-Phospho-Cyclin B1 (Ser133) antibody (TMAB-10559)
Dilution: 1 μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody (white blue line): F (ab’)2 fragment goat anti-rabbit IgG-FITC
Dilution: 1 μg /test.
Protocol
The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 0.1% P000 events was performed.

ICC/IF: 1:100-500; FCM: 1μg/Test

Polyclonal

KLH conjugated Synthesised phosphopeptide: human Cyclin B1 around the phosphorylation site of Ser133

Human

CCNB1

G2/mitotic-specific cyclin-B1

Essential for the control of the cell cycle at the G2/M (mitosis) transition.