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Anti-Phospho-Cyclin B1 (Ser126) Polyclonal Antibody
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Catalog No. TMAB-10558
Anti-Phospho-Cyclin B1 (Ser126) Polyclonal Antibody is a Rabbit antibody targeting Phospho-Cyclin B1 (Ser126). Anti-Phospho-Cyclin B1 (Ser126) Polyclonal Antibody can be used in IHC-P,IHC-Fr,ICC/IF,IF.
Anti-Phospho-Cyclin B1 (Ser126) Polyclonal Antibody
Anti-Phospho-Cyclin B1 (Ser126) Polyclonal Antibody
Copy Product Info😃Good
Catalog No. TMAB-10558
Anti-Phospho-Cyclin B1 (Ser126) Polyclonal Antibody is a Rabbit antibody targeting Phospho-Cyclin B1 (Ser126). Anti-Phospho-Cyclin B1 (Ser126) Polyclonal Antibody can be used in IHC-P,IHC-Fr,ICC/IF,IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $223 | 7-10 days | 7-10 days | |
| 100 μL | $373 | 7-10 days | 7-10 days | |
| 200 μL | $527 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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| Description | Anti-Phospho-Cyclin B1 (Ser126) Polyclonal Antibody is a Rabbit antibody targeting Phospho-Cyclin B1 (Ser126). Anti-Phospho-Cyclin B1 (Ser126) Polyclonal Antibody can be used in IHC-P,IHC-Fr,ICC/IF,IF. |
| Ig Type | IgG |
| Reactivity | Human |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (Human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Phospho-Cyclin B1 (Ser126)) Polyclonal Antibody, Unconjugated (TMAB-10558) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded (Human esophageal carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Phospho-Cyclin B1 (Ser126)) Polyclonal Antibody, Unconjugated (TMAB-10558) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded (human skin cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Phospho-Cyclin B1 (Ser126)) Polyclonal Antibody, Unconjugated (TMAB-10558) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. 4. Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (Phospho-Cyclin B1 (Ser126)) polyclonal Antibody, Unconjugated (TMAB-10558) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei. |
| Application | |
| Recommended Dose | IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, centrosome. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. [provided by RefSeq, Jul 2008]. |
| Immunogen | KLH conjugated synthesised phosphopeptide: human Cyclin B1 around the phosphorylation site of Ser126 |
| Antigen Species | Human |
| Gene Name | CCNB1 |
| Gene ID | |
| Protein Name | G2/mitotic-specific cyclin-B1 |
| Uniprot ID | |
| Function | Essential for the control of the cell cycle at the G2/M (mitosis) transition. |
| Molecular Weight | Theoretical: 48 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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