Anti-Phospho-CXCR4 (Ser339) Polyclonal Antibody 2 is a Rabbit antibody targeting Phospho-CXCR4 (Ser339). Anti-Phospho-CXCR4 (Ser339) Polyclonal Antibody 2 can be used in WB,FCM.

IgG

Mouse (predicted:Human,Rat,Dog,Cow,Horse,Rabbit)

1. Blank control (mouse): Mouse Spleen Cells (fixed with 2% paraformaldehyde for 10 min at 37℃). Primary Antibody: Rabbit Anti-phospho-CXCR4 (Ser339) antibody (TMAB-10557, Green); Dilution: 1 μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions; Secondary Antibody: Goat anti-rabbit IgG-FITC (white blue), Dilution: 1: 200 in 1 X PBS containing 0.5% BSA.
2. Sample:
Lane 1: Lung (Mouse) Lysate at 40 μg
Lane 2: Spleen (Mouse) Lysate at 40 μg
Lane 3: Uterus (Mouse) Lysate at 40 μg
Lane 4: Lymph node (Mouse) Lysate at 40 μg
Primary: Anti-phospho-CXCR4 (Ser339) (TMAB-10557) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 45 kD
Observed band size: 50 kD

WB: 1:500-2000; FCM: 1μg/Test

Polyclonal

KLH conjugated Synthesised phosphopeptide: human CXCR4 around the phosphorylation site of Ser339

Human

CXCR4

C-X-C chemokine receptor type 4

Receptor for the C-X-C chemokine CXCL12/SDF-1 that transduces a signal by increasing intracellular calcium ions levels and enhancing MAPK1/MAPK3 activation. Acts as a receptor for extracellular ubiquitin; leading to enhance intracellular calcium ions and reduce cellular cAMP levels. Involved in haematopoiesis and in cardiac ventricular septum formation. Plays also an essential role in vascularization of the gastrointestinal tract, probably by regulating vascular branching and/or remodeling processes in endothelial cells. Could be involved in cerebellar development. In the CNS, could mediate hippocampal-neuron survival. Acts as a coreceptor (CD4 being the primary receptor) for HIV-1 X4 isolates and as a primary receptor for some HIV-2 isolates. Promotes Env-mediated fusion of the virus.