Anti-Phospho-CTNNA1 (Ser641) Polyclonal Antibody is a Rabbit antibody targeting Phospho-CTNNA1 (Ser641). Anti-Phospho-CTNNA1 (Ser641) Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.

IgG

Mouse (predicted:Human,Rat,Chicken,Dog,Pig,Cow,Horse,Rabbit,Sheep)

Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CTNNA1 (Ser641)) Polyclonal Antibody, Unconjugated (TMAB-10554) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.

IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated Synthesised phosphopeptide: human CTNNA1 around the phosphorylation site of Ser641

Human

CTNNA1

Catenin alpha-1

Associates with the cytoplasmic domain of a variety of cadherins. The association of catenins to cadherins produces a complex which is linked to the actin filament network, and which seems to be of primary importance for cadherins cell-adhesion properties. Can associate with both E- and N-cadherins. Originally believed to be a stable component of E-cadherin/catenin adhesion complexes and to mediate the linkage of cadherins to the actin cytoskeleton at adherens junctions. In contrast, cortical actin was found to be much more dynamic than E-cadherin/catenin complexes and CTNNA1 was shown not to bind to F-actin when assembled in the complex suggesting a different linkage between actin and adherens junctions components. The homodimeric form may regulate actin filament assembly and inhibit actin branching by competing with the Arp2/3 complex for binding to actin filaments. May play a crucial role in cell differentiation.