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Anti-Phospho-CstF64 (Ser83) Polyclonal Antibody
Catalog No. TMAB-10552 Copy Product Info
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Anti-Phospho-CstF64 (Ser83) Polyclonal Antibody is a Rabbit antibody targeting Phospho-CstF64 (Ser83). Anti-Phospho-CstF64 (Ser83) Polyclonal Antibody can be used in WB.
Anti-Phospho-CstF64 (Ser83) Polyclonal Antibody
Copy Product Info🥰Excellent
Catalog No. TMAB-10552
Anti-Phospho-CstF64 (Ser83) Polyclonal Antibody is a Rabbit antibody targeting Phospho-CstF64 (Ser83). Anti-Phospho-CstF64 (Ser83) Polyclonal Antibody can be used in WB.
Anti-Phospho-CstF64
(Ser83) Polyclonal Antibody
(Ser83) Polyclonal Antibody
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 50 μL | $220 | 7-10 days | 7-10 days | |
| 100 μL | $374 | 7-10 days | 7-10 days | |
| 200 μL | $527 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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| Description | Anti-Phospho-CstF64 (Ser83) Polyclonal Antibody is a Rabbit antibody targeting Phospho-CstF64 (Ser83). Anti-Phospho-CstF64 (Ser83) Polyclonal Antibody can be used in WB. |
| Ig Type | IgG |
| Reactivity | Human (predicted:Mouse,Rat,Chicken,Dog,Pig,Cow,Horse,Rabbit,Sheep) |
| Verified Activity | Sample: MCF-7 Cell (Human) Lysate at 40 μg Primary: Anti-phospho-CstF64 (Ser83) (TMAB-10552) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 61 kD Observed band size: 61 kD |
| Application | |
| Recommended Dose | WB: 1:500-2000 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Nuclear |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | Polyadenylation of mRNA precursors is a two-step reaction that requires multiple protein factors. The first step, endonucleolytic cleavage of polyadenylation substrates, requires CstF (cleavage stimulation factor), a heterotrimer that is composed of three distinct subunits. CstF-64 contains an RNA binding domain and is responsible for the RNA binding activity of CstF. CstF-64 is expressed in all somatic cells and in pre- and postmeiotic, but not meiotic, germ cells. However, a large variant of CstF-64, called t CstF-64, is abundantly expressed in meiotic and postmeiotic cells in the testis and to a lesser extent in the brain, and promotes the germ cell pattern of polyadenylation. The gene encoding CstF-64 (designated CSTF2) maps to the X chromosome, whereas t CstF-64 is encoded by an autosomal gene. The increase in CstF-64 concentration during B cell activation switches IgM heavy chain mRNA expression from membrane-bound to secreted forms, suggesting that CstF-64 plays a key role in regulating IgM heavy chain expression during B cell differentiation. |
| Immunogen | KLH conjugated synthesised phosphopeptide: human CstF64 around the phosphorylation site of Ser83 |
| Antigen Species | Human |
| Gene Name | CSTF2 |
| Gene ID | |
| Protein Name | Cleavage stimulation factor subunit 2 |
| Uniprot ID | |
| Function | One of the multiple factors required for polyadenylation and 3'-end cleavage of mammalian pre-mRNAs. This subunit is directly involved in the binding to pre-mRNAs (By similarity). |
| Molecular Weight | Theoretical: 61 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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