Anti-Phospho-CSNK1G 1+2+3 (Tyr263) Polyclonal Antibody is a Rabbit antibody targeting Phospho-CSNK1G 1+2+3 (Tyr263). Anti-Phospho-CSNK1G 1+2+3 (Tyr263) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Mouse,Rat (predicted:Human,Dog,Pig,Cow,Horse)

1. Sample: Bone (Mouse) Lysate at 40 μg
Primary: Anti-phospho-CSNK1G 1+2+3 (Tyr263) (TMAB-10551) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 49 kD
Observed band size: 49 kD
2. Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CSNK1G 1+2+3 (Tyr263)) Polyclonal Antibody, Unconjugated (TMAB-10551) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
3. Sample: Lymph node (Mouse) Lysate at 40 μg
Primary: Anti-phospho-CSNK1G 1+2+3 (Tyr263) (TMAB-10551) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 49 kD
Observed band size: 49 kD
4. Sample: Brain (Mouse) Lysate at 40 μg
Primary: Anti-phospho-CSNK1G 1+2+3 (Tyr263) (TMAB-10551) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 49 kD
Observed band size: 49 kD

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthesised phosphopeptide: human Casein kinase I isoforms gamma 1+2+3 around the phosphorylation site of Tyr263

Human

CSNK1G1

Casein kinase I isoform gamma-1

Serine/threonine-protein kinase. Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Regulates fast synaptic transmission mediated by glutamate (By similarity). Phosphorylates CLSPN.