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Anti-Phospho-Crkl (Tyr251) Polyclonal Antibody
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Catalog No. TMAB-10545
Anti-Phospho-Crkl (Tyr251) Polyclonal Antibody is a Rabbit antibody targeting Phospho-Crkl (Tyr251). Anti-Phospho-Crkl (Tyr251) Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
Anti-Phospho-Crkl (Tyr251) Polyclonal Antibody
Anti-Phospho-Crkl (Tyr251) Polyclonal Antibody
Copy Product Info😃Good
Catalog No. TMAB-10545
Anti-Phospho-Crkl (Tyr251) Polyclonal Antibody is a Rabbit antibody targeting Phospho-Crkl (Tyr251). Anti-Phospho-Crkl (Tyr251) Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $221 | 7-10 days | 7-10 days | |
| 100 μL | $373 | 7-10 days | 7-10 days | |
| 200 μL | $529 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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| Description | Anti-Phospho-Crkl (Tyr251) Polyclonal Antibody is a Rabbit antibody targeting Phospho-Crkl (Tyr251). Anti-Phospho-Crkl (Tyr251) Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Human (predicted:Mouse,Rat,Dog,Pig,Cow,Horse,Rabbit,Sheep) |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (phospho-Crkl (Tyr251)) Polyclonal Antibody, Unconjugated (TMAB-10545) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded (human colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (phospho-Crkl (Tyr251)) Polyclonal Antibody, Unconjugated (TMAB-10545) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded (human stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (phospho-Crkl (Tyr251)) Polyclonal Antibody, Unconjugated (TMAB-10545) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (phospho-Crkl (Tyr251)) Polyclonal Antibody, Unconjugated (TMAB-10545) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. |
| Application | |
| Recommended Dose | IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | Crkl is a 34kDa adaptor protein which has been shown to activate the RAS and JUN kinase signalling pathways and transforms fibroblasts in a RAS dependant manner. Crkl is a substitute of BCR ABL tyrosine kinase. In addition, Crkl has oncogenic potential. |
| Immunogen | KLH conjugated Synthesised phosphopeptide: human Crkl around the phosphorylation site of Tyr251 |
| Antigen Species | Human |
| Gene Name | CRKL |
| Gene ID | |
| Protein Name | Crk-like protein |
| Uniprot ID | |
| Function | May mediate the transduction of intracellular signals. |
| Molecular Weight | Theoretical: 34 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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