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Anti-Phospho-CREM (Ser271+Ser274) Polyclonal Antibody
Catalog No. TMAB-10542 Copy Product Info
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Anti-Phospho-CREM (Ser271+Ser274) Polyclonal Antibody is a Rabbit antibody targeting Phospho-CREM (Ser271+Ser274). Anti-Phospho-CREM (Ser271+Ser274) Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
Anti-Phospho-CREM (Ser271+Ser274) Polyclonal Antibody
Copy Product Info🥰Excellent
Catalog No. TMAB-10542
Anti-Phospho-CREM (Ser271+Ser274) Polyclonal Antibody is a Rabbit antibody targeting Phospho-CREM (Ser271+Ser274). Anti-Phospho-CREM (Ser271+Ser274) Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
Anti-Phospho-CREM
(Ser271+Ser274) Polyclonal Antibody
(Ser271+Ser274) Polyclonal Antibody
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 50 μL | $220 | 7-10 days | 7-10 days | |
| 100 μL | $372 | 7-10 days | 7-10 days | |
| 200 μL | $529 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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| Description | Anti-Phospho-CREM (Ser271+Ser274) Polyclonal Antibody is a Rabbit antibody targeting Phospho-CREM (Ser271+Ser274). Anti-Phospho-CREM (Ser271+Ser274) Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Mouse,Rat (predicted:Human,Chicken,Pig,Horse) |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (phospho-CREM (Ser271 +Ser 274)) Polyclonal Antibody, Unconjugated (TMAB-10542) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded (mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (phospho-CREM (Ser271 +Ser 274)) Polyclonal Antibody, Unconjugated (TMAB-10542) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. |
| Application | |
| Recommended Dose | IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Nucleus. |
| Tissue Specificity | Expressed in testes (round spermatids) (at protein level). Isoform 14 is the major activator form in testes. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | CREM is a a bZIP transcription factor that binds to the cAMP responsive element found in many viral and cellular promoters. It is derived from a multiexonic gene that encodes both activators and antagonists of cAMP-inducible transcription by differential splicing. Splice variants with antagonistic function lack 2 glutamine-rich domains and block cAMP-induced transcription, whereas an isoform that includes these glutamine-rich domains is a transcriptional activator. |
| Immunogen | KLH conjugated Synthesised phosphopeptide: human CREM around the phosphorylation site of Ser271 + Ser274 |
| Antigen Species | Human |
| Gene Name | CREM |
| Gene ID | |
| Protein Name | cAMP-responsive element modulator |
| Uniprot ID | |
| Function | Transcriptional regulator that binds the cAMP response element (CRE), a sequence present in many viral and cellular promoters. Isoforms are either transcriptional activators or repressors. Plays a role in spermatogenesis and is involved in spermatid maturation. |
| Molecular Weight | Theoretical: 37 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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