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Anti-Phospho-CK1 alpha (Tyr294) Polyclonal Antibody
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Catalog No. TMAC-00870
Alias p-CK1 alpha (Y294), p-CK1 alpha (Tyr294), CK1 alpha (p-Y294), CK1 alpha (p-Tyr294)
Anti-Phospho-CK1 alpha (Tyr294) Polyclonal Antibody is a Rabbit antibody targeting Phospho-CK1 alpha (Tyr294). Anti-Phospho-CK1 alpha (Tyr294) Polyclonal Antibody can be used in IHC,WB.
Anti-Phospho-CK1 alpha (Tyr294) Polyclonal Antibody
Anti-Phospho-CK1 alpha (Tyr294) Polyclonal Antibody
😃Good
Catalog No. TMAC-00870Alias p-CK1 alpha (Y294), p-CK1 alpha (Tyr294), CK1 alpha (p-Y294), CK1 alpha (p-Tyr294)
Anti-Phospho-CK1 alpha (Tyr294) Polyclonal Antibody is a Rabbit antibody targeting Phospho-CK1 alpha (Tyr294). Anti-Phospho-CK1 alpha (Tyr294) Polyclonal Antibody can be used in IHC,WB.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $217 | 7-10 days | 7-10 days | |
| 100 μL | $316 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Anti-Phospho-CK1 alpha (Tyr294) Polyclonal Antibody is a Rabbit antibody targeting Phospho-CK1 alpha (Tyr294). Anti-Phospho-CK1 alpha (Tyr294) Polyclonal Antibody can be used in IHC,WB. |
| Synonyms | p-CK1 alpha (Y294), p-CK1 alpha (Tyr294), CK1 alpha (p-Y294), CK1 alpha (p-Tyr294) |
| Ig Type | IgG |
| Reactivity | Human |
| Verified Activity | 1. Western blot analysis of extracts from HT-29 cells treated with heat shock using CK-1α (Phospho-Tyr294) Antibody TMAC-00870.The lane on the right is treated with the antigen-specific peptide. 2. Immunohistochemical analysis of paraffin-embedded human brain tissue using CK-1α (Phospho-Tyr294) antibody TMAC-00870 (left)or the same antibody preincubated with blocking peptide (right).   |
| Application | |
| Recommended Dose | WB: 1:500-1000; IHC: 1:50-100 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Construction | Polyclonal Antibody |
| Purification | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| Appearance | Liquid |
| Formulation | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Research Background | Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates CTNNB1 at 'Ser-45'. May play a role in segregating chromosomes during mitosis. |
| Conjucates | Unconjugated |
| Others Formats | Phospho |
| Immunogen | Peptide sequence around phosphorylation site of tyrosine 294 (Y-D-Y(p)-T-F) derived from Human CK-1α |
| Antigen Species | human |
| Uniprot ID |
| Molecular Weight | Actual: 37 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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