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Anti-Phospho-Chk2 (Thr68) Antibody (7T986)

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Catalog No. TMAY-01664
Alias TPDS4, RAD53, PP1425, LFS2, HuCds1, hCds1, CHK-2, CDS1, bA444G7

Anti-Phospho-Chk2 (Thr68) Antibody (7T986) is a Rabbit antibody targeting Phospho-Chk2 (Thr68). Anti-Phospho-Chk2 (Thr68) Antibody (7T986) can be used in WB.

Anti-Phospho-Chk2 (Thr68) Antibody (7T986)

Anti-Phospho-Chk2 (Thr68) Antibody (7T986)

😃Good
Catalog No. TMAY-01664Alias TPDS4, RAD53, PP1425, LFS2, HuCds1, hCds1, CHK-2, CDS1, bA444G7
Anti-Phospho-Chk2 (Thr68) Antibody (7T986) is a Rabbit antibody targeting Phospho-Chk2 (Thr68). Anti-Phospho-Chk2 (Thr68) Antibody (7T986) can be used in WB.
Pack SizePriceAvailabilityQuantity
20 μL$80 7-10 days
100 μL$235 7-10 days
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Product Introduction

Bioactivity
Description
Anti-Phospho-Chk2 (Thr68) Antibody (7T986) is a Rabbit antibody targeting Phospho-Chk2 (Thr68). Anti-Phospho-Chk2 (Thr68) Antibody (7T986) can be used in WB.
AliasTPDS4, RAD53, PP1425, LFS2, HuCds1, hCds1, CHK-2, CDS1, bA444G7
Ig Type
Monoclonal Rabbit IgG
Clone
7T986
Reactivity
Human
Verified Activity
1. Western blot analysis of extracts from HCT116, untreated (-) or treated with Etoposide (100 μM, 2 h) (+), using Phospho-Chk2 (Thr68) Antibody, Rabbit MAb at 1:500 dilution (upper) or Anti-CHEK2 Antibody, Rabbit PAb (middle) at 1:1000 dilution or Beta-Tubulin Loading Control Antibody, Mouse Mab at 1:10000 dilution.

2. Western blot analysis of extracts from HCT116, untreated (line A); treated with Etoposide (100 μM, 2 h), without peptide (line B) or antigen-specific phosphopeptide (line C) or antigen-specific peptide (line D) using Phospho-Chk2 (Thr68) Antibody, Rabbit MAb at 1:500 dilution. (Validation Experiment)

3. Western blot analysis of extracts from HCT116, untreated (line A); treated with Etoposide (100 μM, 2 h) (line B); treated with Etoposide and λ-phosphatase (line C) using Phospho-Chk2 (Thr68) Antibody, Rabbit MAb at 1:500 dilution. (Validation Experiment)

4. Western blot analysis of extracts from HCT116, treated with Etoposide (100μM, 2 h), using Phospho-Chk2 (Thr68) Antibody, Rabbit MAb and other brands’ antibodies (Company C) at dilution of 1:1000, 1:5000. (Validation Experiment)
Application
WB
Recommended Dose
WB: 1:1000-1:10000
Antibody Type
Monoclonal
Host SpeciesRabbit
ConstructionThis antibody was obtained from a rabbit immunized with a synthetic phosphopeptide corresponding to residues around Thr68 of the Human Chk2.
PurificationProtein A
AppearanceLiquid
Formulation10 mM sodium HEPES, 150 mM NaCl, 100 μg/mL BSA, 50% glycerol, pH 7.5
Research BackgroundIn response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by CHEK2 gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded CHEK2 protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in CHEK2s gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Several transcript variants encoding different isoforms have been found for this gene.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
A synthetic phosphopeptide corresponding to residues around Thr68 of the Human Chk2
Antigen Species
Human
Biology Area
Cancer Drug Targets, Tumor Suppressors
Chemical Properties
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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