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Anti-Phospho-Chk1 (Ser345) Antibody (8E317)
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Catalog No. TMAY-01574
Alias Phospho-Chk1 (S345), p-Chk1 (Ser345), p-Chk1 (S345), OZEMA21, Chk1 (p-Ser345), Chk1 (p-S345), CHK-1
Anti-Phospho-Chk1 (Ser345) Antibody (8E317) is a Rabbit antibody targeting Phospho-Chk1 (Ser345). Anti-Phospho-Chk1 (Ser345) Antibody (8E317) can be used in WB.
Anti-Phospho-Chk1 (Ser345) Antibody (8E317)
Anti-Phospho-Chk1 (Ser345) Antibody (8E317)
😃Good
Catalog No. TMAY-01574Alias Phospho-Chk1 (S345), p-Chk1 (Ser345), p-Chk1 (S345), OZEMA21, Chk1 (p-Ser345), Chk1 (p-S345), CHK-1
Anti-Phospho-Chk1 (Ser345) Antibody (8E317) is a Rabbit antibody targeting Phospho-Chk1 (Ser345). Anti-Phospho-Chk1 (Ser345) Antibody (8E317) can be used in WB.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 20 μL | $82 | 7-10 days | |
| 100 μL | $233 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Rabbit Monoclonal Application: WB Reactivity: Human |
| Alias | Phospho-Chk1 (S345), p-Chk1 (Ser345), p-Chk1 (S345), OZEMA21, Chk1 (p-Ser345), Chk1 (p-S345), CHK-1 |
| Ig Type | Rabbit IgG |
| Clone | 8E317 |
| Reactivity | Human |
| Verified Activity | 1. Western blot analysis of extracts from serum-starved Hela, untreated (-) or treated with Calyculin A (100 nM, 30 min; +), using Phospho-Chk1 (Ser345) rabbit monoclonal Antibody at 1:5000 dilution (upper) or Anti-beta-Actin Antibody Loading Control Antibody, Rabbit MAb (Chimeric) at 1:50000 dilution (lower). 2. Western blot analysis of extracts from serum-starved Hela, untreated (line A); treated with Calyculin A (100 nM, 30 min), without peptide (line B) or antigen-specific phosphopeptide (line C) or antigen-specific peptide (line D) using Phospho-Chk1 (Ser345) rabbit monoclonal Antibody at 1:1000 dilution. (Validation Experiment) 3. Western blot analysis of extracts from serum-starved Hela, untreated (line A); treated with calyculin A (100 nM, 30min; +) (line B); treated with calyculin A and λ-phosphatase (line C) using Phospho-Chk1 (Ser345) rabbit monoclonal Antibody at 1:1000 dilution. (Validation Experiment) |
| Application | WB |
| Recommended Dose | WB: 1:2000-1:20000 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | This antibody was obtained from a rabbit immunized with a synthetic peptide corresponding to residues around (Ser345) of Human Phospho-Chk1. |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 10 mM sodium HEPES, 150 mM NaCl, 100 μg/mL BSA, 50% glycerol, pH 7.5 |
| Research Background | CHK1 / CHEK1 contains 1 protein kinase domain and belongs to the protein kinase superfamily, CAMK Ser/Thr protein kinase family, NIM1 subfamily. It is a member of checkpoint kinases (Chks). Chks Checkpoint kinases (Chks) are serine/threonine kinases that are involved in the control of the cell cycle. There are two subtypes of chks that have so far been identified, CHK1 / CHEK1 and Chk2. They are essential components to delay cell cycle progression in normal and damaged cells and can act at all three cell cycle checkpoints. Chks are activated by phosphorylation. ATR kinase phosphorylates CHK1 / CHEK1 in response to single strand DNA breaks and ATM kinase phosphorylates Chk2 in response to double strand breaks. Chks phosphorylate Cdc25 phosphatase at Ser216, which leads to Cdc25 sequestration in the cytoplasm. Chks have a role in the physiological stress of hypoxia/reoxygenation. CHK1 / CHEK1 is required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. CHK1 / CHEK1 may also negatively regulate cell cycle progression during unperturbed cell cycles.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy |
| Conjucates | Unconjugated |
| Immunogen | A synthetic peptide corresponding to residues around (Ser345) of Human Phospho-Chk1 |
| Antigen Species | Human |
| Biology Area | Cancer Drug Targets, Tumor Suppressors |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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