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Anti-Phospho-Chk1 (Ser345) Antibody (8E317)

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Catalog No. TMAY-01574
Alias OZEMA21, CHK-1

Anti-Phospho-Chk1 (Ser345) Antibody (8E317) is a Rabbit antibody targeting Phospho-Chk1 (Ser345). Anti-Phospho-Chk1 (Ser345) Antibody (8E317) can be used in WB.

Anti-Phospho-Chk1 (Ser345) Antibody (8E317)

Anti-Phospho-Chk1 (Ser345) Antibody (8E317)

😃Good
Catalog No. TMAY-01574Alias OZEMA21, CHK-1
Anti-Phospho-Chk1 (Ser345) Antibody (8E317) is a Rabbit antibody targeting Phospho-Chk1 (Ser345). Anti-Phospho-Chk1 (Ser345) Antibody (8E317) can be used in WB.
Pack SizePriceAvailabilityQuantity
20 μL$82 7-10 days
100 μL$233 7-10 days
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Product Introduction

Bioactivity
Description
Anti-Phospho-Chk1 (Ser345) Antibody (8E317) is a Rabbit antibody targeting Phospho-Chk1 (Ser345). Anti-Phospho-Chk1 (Ser345) Antibody (8E317) can be used in WB.
AliasOZEMA21, CHK-1
Ig Type
Monoclonal Rabbit IgG
Clone
8E317
Reactivity
Human
Verified Activity
1. Western blot analysis of extracts from serum-starved Hela, untreated (-) or treated with Calyculin A (100 nM, 30 min; +), using Phospho-Chk1 (Ser345) rabbit monoclonal Antibody at 1:5000 dilution (upper) or Anti-beta-Actin Antibody Loading Control Antibody, Rabbit MAb (Chimeric) at 1:50000 dilution (lower).

2. Western blot analysis of extracts from serum-starved Hela, untreated (line A); treated with Calyculin A (100 nM, 30 min), without peptide (line B) or antigen-specific phosphopeptide (line C) or antigen-specific peptide (line D) using Phospho-Chk1 (Ser345) rabbit monoclonal Antibody at 1:1000 dilution. (Validation Experiment)

3. Western blot analysis of extracts from serum-starved Hela, untreated (line A); treated with calyculin A (100 nM, 30min; +) (line B); treated with calyculin A and λ-phosphatase (line C) using Phospho-Chk1 (Ser345) rabbit monoclonal Antibody at 1:1000 dilution. (Validation Experiment)
Application
WB
Recommended Dose
WB: 1:2000-1:20000
Antibody Type
Monoclonal
Host SpeciesRabbit
ConstructionThis antibody was obtained from a rabbit immunized with a synthetic peptide corresponding to residues around (Ser345) of Human Phospho-Chk1.
PurificationProtein A
AppearanceLiquid
Formulation10 mM sodium HEPES, 150 mM NaCl, 100 μg/mL BSA, 50% glycerol, pH 7.5
Research BackgroundCHK1 / CHEK1 contains 1 protein kinase domain and belongs to the protein kinase superfamily, CAMK Ser/Thr protein kinase family, NIM1 subfamily. It is a member of checkpoint kinases (Chks). Chks Checkpoint kinases (Chks) are serine/threonine kinases that are involved in the control of the cell cycle. There are two subtypes of chks that have so far been identified, CHK1 / CHEK1 and Chk2. They are essential components to delay cell cycle progression in normal and damaged cells and can act at all three cell cycle checkpoints. Chks are activated by phosphorylation. ATR kinase phosphorylates CHK1 / CHEK1 in response to single strand DNA breaks and ATM kinase phosphorylates Chk2 in response to double strand breaks. Chks phosphorylate Cdc25 phosphatase at Ser216, which leads to Cdc25 sequestration in the cytoplasm. Chks have a role in the physiological stress of hypoxia/reoxygenation. CHK1 / CHEK1 is required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. CHK1 / CHEK1 may also negatively regulate cell cycle progression during unperturbed cell cycles.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
A synthetic peptide corresponding to residues around (Ser345) of Human Phospho-Chk1
Antigen Species
Human
Biology Area
Cancer Drug Targets, Tumor Suppressors
Chemical Properties
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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