Anti-Phospho-CEBP alpha (Ser21) Polyclonal Antibody is a Rabbit antibody targeting Phospho-CEBP alpha (Ser21). Anti-Phospho-CEBP alpha (Ser21) Polyclonal Antibody can be used in ICC/IF.

IgG

Human (predicted:Mouse,Rat,Pig,Cow,Rabbit)

1. HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (Phospho-CEBP alpha (Ser21)) polyclonal Antibody, Unconjugated (TMAB-10487) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.
2. HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (Phospho-CEBP alpha (Ser21)) polyclonal Antibody, Unconjugated (TMAB-10487) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

ICC/IF: 1:50-200

Polyclonal

KLH conjugated Synthesised phosphopeptide: human CDBP alpha around the phosphorylation site of Ser21

Human

CEBPA

CCAAT/enhancer-binding protein alpha

C/EBP is a DNA-binding protein that recognizes two different motifs: the CCAAT homology common to many promoters and the enhanced core homology common to many enhancers.