Anti-Phospho-CDKN1B (Ser178) Polyclonal Antibody 2 is a Rabbit antibody targeting Phospho-CDKN1B (Ser178). Anti-Phospho-CDKN1B (Ser178) Polyclonal Antibody 2 can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Mouse,Rat (predicted:Dog,Pig,Cow,Horse)

1. Tissue/cell: mouse intestine tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-phospho-CDKN1b (Ser178) Polyclonal Antibody, Unconjugated 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
2. Sample:
Lane 1: NIH/3T3 (Mouse) Cell Lysate at 30 μg
Lane 2: Spleen (Rat) Lysate at 40 μg
Lane 3: Thymus (Rat) Lysate at 40 μg
Lane 4: Hela (Human) Cell Lysate at 30 μg
Lane 5: SH-SY5Y (Human) Cell Lysate at 30 μg
Lane 6: THP-1 (Human) Cell Lysate at 30 μg
Lane 7: HepG2 (Human) Cell Lysate at 30 μg
Lane 8: MCF-7 (Human) Cell Lysate at 30 μg
Primary: Anti-phospho-CDKN1B (Ser178) (TMAB-10482) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 27 kD
Observed band size: 27 kD
3. Paraformaldehyde-fixed, paraffin embedded (mouse skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (phospho-CDKN1B (Ser178)) Polyclonal Antibody, Unconjugated (TMAB-10482) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded (rat skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (phospho-CDKN1B (Ser178)) Polyclonal Antibody, Unconjugated (TMAB-10482) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated Synthesised phosphopeptide: human CDKN1B around the phosphorylation site of Ser178

Human

CDKN1B

Cyclin-dependent kinase inhibitor 1B

Important regulator of cell cycle progression. Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry.