Anti-Phospho-CDC37 (Ser13) Antibody (4R745) is a Rabbit antibody targeting Phospho-CDC37 (Ser13). Anti-Phospho-CDC37 (Ser13) Antibody (4R745) can be used in WB.

IgG

4R745

Human,Mouse,Rat

1. Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1: 2000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP)
Lysate: (-): HeLa, (+): HeLa+Okadaic A (100 nM,60 min)+Calyculin A (100 nM,60 min)
Protein loading quantity: 20 μg
Exposure time: 30 s
Predicted MW: 50 kDa
Observed MW: 50 kDa
2. Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1: 2000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP)
Lysate: （-): C6, (+): C6+Calyculin A (100 nM, 30 min)
Protein loading quantity: 20 μg
Exposure time: 30 s
Predicted MW: 50 kDa
Observed MW: 50 kDa

WB: 1:1000-2000

Monoclonal

KLH conjugated synthesised phosphopeptide: human CDC37 around the phosphorylation site of Ser13

Human

CDC37

Hsp90 co-chaperone Cdc37

Co-chaperone that binds to numerous kinases and promotes their interaction with the Hsp90 complex, resulting in stabilization and promotion of their activity.