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Anti-Phospho-CDC25C (Ser216) Polyclonal Antibody
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Catalog No. TMAC-00765
Alias p-CDC25C (Ser216), p-CDC25C (S216), CDC25C (p-Ser216), CDC25C (p-S216)
Anti-Phospho-CDC25C (Ser216) Polyclonal Antibody is a Rabbit antibody targeting Phospho-CDC25C (Ser216). Anti-Phospho-CDC25C (Ser216) Polyclonal Antibody can be used in IF,IHC,WB.
Anti-Phospho-CDC25C (Ser216) Polyclonal Antibody
Anti-Phospho-CDC25C (Ser216) Polyclonal Antibody
😃Good
Catalog No. TMAC-00765Alias p-CDC25C (Ser216), p-CDC25C (S216), CDC25C (p-Ser216), CDC25C (p-S216)
Anti-Phospho-CDC25C (Ser216) Polyclonal Antibody is a Rabbit antibody targeting Phospho-CDC25C (Ser216). Anti-Phospho-CDC25C (Ser216) Polyclonal Antibody can be used in IF,IHC,WB.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $218 | 7-10 days | 7-10 days | |
| 100 μL | $317 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Anti-Phospho-CDC25C (Ser216) Polyclonal Antibody is a Rabbit antibody targeting Phospho-CDC25C (Ser216). Anti-Phospho-CDC25C (Ser216) Polyclonal Antibody can be used in IF,IHC,WB. |
| Synonyms | p-CDC25C (Ser216), p-CDC25C (S216), CDC25C (p-Ser216), CDC25C (p-S216) |
| Ig Type | IgG |
| Reactivity | Human |
| Verified Activity | 1. Western blot analysis of extracts from 293 cells untreated or treated with serum using cdc25C (Phospho-Ser216) Antibody TMAC-00765. 2. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using cdc25C (Phospho-Ser216) Antibody TMAC-00765 (left) or the same antibody preincubated with blocking peptide (right). 3. Immunofluorescence staining of methanol-fixed Hela cells using cdc25C (Phospho-Ser216) Antibody TMAC-00765.    |
| Application | |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Construction | Polyclonal Antibody |
| Purification | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| Appearance | Liquid |
| Formulation | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Research Background | cdc25C is highly conserved during evolution and it plays a key role in the regulation of cell division. The encoded protein is a tyrosine phosphatase and belongs to the Cdc25 phosphatase family. It directs dephosphorylation of cyclin B-bound CDC2 and triggers entry into mitosis. It is also thought to suppress p53-induced growth arrest. Multiple alternatively spliced transcript variants of this gene have been described, however, the full-length nature of many of them is not known. |
| Conjucates | Unconjugated |
| Others Formats | Phospho |
| Immunogen | Peptide sequence around phosphorylation site of serine 216 (S-P-S(p)-M-P) derived from Human cdc25C |
| Antigen Species | human |
| Uniprot ID |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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