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Anti-Phospho-CDC25B (Ser323) Polyclonal Antibody
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Catalog No. TMAC-00760
Alias p-CDC25B (Ser323), p-CDC25B (S323), CDC25B (p-Ser323), CDC25B (p-S323)
Anti-Phospho-CDC25B (Ser323) Polyclonal Antibody is a Rabbit antibody targeting Phospho-CDC25B (Ser323). Anti-Phospho-CDC25B (Ser323) Polyclonal Antibody can be used in WB.
Anti-Phospho-CDC25B (Ser323) Polyclonal Antibody
Anti-Phospho-CDC25B (Ser323) Polyclonal Antibody
😃Good
Catalog No. TMAC-00760Alias p-CDC25B (Ser323), p-CDC25B (S323), CDC25B (p-Ser323), CDC25B (p-S323)
Anti-Phospho-CDC25B (Ser323) Polyclonal Antibody is a Rabbit antibody targeting Phospho-CDC25B (Ser323). Anti-Phospho-CDC25B (Ser323) Polyclonal Antibody can be used in WB.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $216 | 7-10 days | 7-10 days | |
| 100 μL | $318 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Anti-Phospho-CDC25B (Ser323) Polyclonal Antibody is a Rabbit antibody targeting Phospho-CDC25B (Ser323). Anti-Phospho-CDC25B (Ser323) Polyclonal Antibody can be used in WB. |
| Synonyms | p-CDC25B (Ser323), p-CDC25B (S323), CDC25B (p-Ser323), CDC25B (p-S323) |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Western blot analysis of extracts from NIH/3T3 cells treated with PMA (125ng/ml, 30mins), using CDC25B (Phospho-Ser323) antibody TMAC-00760. The lane on the right is treated with the synthesized peptide.  |
| Application | |
| Recommended Dose | WB: 1:500-3000 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Construction | Polyclonal Antibody |
| Purification | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| Appearance | Liquid |
| Formulation | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Research Background | Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Directly dephosphorylates CDK1 and stimulates its kinase activity. The three isoforms seem to have a different level of activity. |
| Conjucates | Unconjugated |
| Others Formats | Phospho |
| Immunogen | Peptide sequence around phosphorylation site of serine 323 (S-P-S(p)-M-P) derived from Human CDC25B |
| Antigen Species | human |
| Uniprot ID |
| Molecular Weight | Actual: 62 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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