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Anti-Phospho-CDC25B (Ser149) Polyclonal Antibody
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Catalog No. TMAC-00763
Alias p-CDC25B (Ser149), p-CDC25B (S149), CDC25B (p-Ser149), CDC25B (p-S149)
Anti-Phospho-CDC25B (Ser149) Polyclonal Antibody is a Rabbit antibody targeting Phospho-CDC25B (Ser149). Anti-Phospho-CDC25B (Ser149) Polyclonal Antibody can be used in WB.
Anti-Phospho-CDC25B (Ser149) Polyclonal Antibody
Anti-Phospho-CDC25B (Ser149) Polyclonal Antibody
😃Good
Catalog No. TMAC-00763Alias p-CDC25B (Ser149), p-CDC25B (S149), CDC25B (p-Ser149), CDC25B (p-S149)
Anti-Phospho-CDC25B (Ser149) Polyclonal Antibody is a Rabbit antibody targeting Phospho-CDC25B (Ser149). Anti-Phospho-CDC25B (Ser149) Polyclonal Antibody can be used in WB.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $217 | 7-10 days | |
| 100 μL | $318 | 7-10 days |
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Chemical Properties
| Description | Anti-Phospho-CDC25B (Ser149) Polyclonal Antibody is a Rabbit antibody targeting Phospho-CDC25B (Ser149). Anti-Phospho-CDC25B (Ser149) Polyclonal Antibody can be used in WB. |
| Synonyms | p-CDC25B (Ser149), p-CDC25B (S149), CDC25B (p-Ser149), CDC25B (p-S149) |
| Ig Type | IgG |
| Reactivity | Mouse |
| Verified Activity | 1. Western blot analysis of extracts from 3T3 cells untreated (lane 1) or treated with Hydroxyurea (lane 2) using Cdc25B (Phospho-Ser149) Antibody TMAC-00763. 2. Western blot analysis of extracts from HT29 cells, treated with PMA or calf intestinal phosphatase (CIP), using Cdc25B (Phospho-Ser149) Antibody TMAC-00763. |
| Application | |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Construction | Polyclonal Antibody |
| Purification | Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide. |
| Appearance | Liquid |
| Formulation | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Research Background | Cdc25B is a member of the CDC25 family of phosphatases. CDC25B activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. CDC25B shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has oncogenic properties, although its role in tumor formation has not been determined. Multiple transcript variants for this gene exist. |
| Conjucates | Unconjugated |
| Others Formats | Phospho |
| Immunogen | Peptide sequence around phosphorylation site of Serine 149 (F-R-S(p)-L-P) derived from Mouse Cdc25B |
| Antigen Species | mouse |
| Uniprot ID |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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