Anti-Phospho-CD244 (Tyr271) Polyclonal Antibody is a Rabbit antibody targeting Phospho-CD244 (Tyr271). Anti-Phospho-CD244 (Tyr271) Polyclonal Antibody can be used in WB,FCM.

IgG

Human (predicted:Pig,Horse,Rabbit)

1. Sample: Hela (Human) Lysate at 40 μg
Primary: Anti-phospho-CD244 (Tyr271) (TMAB-10451) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 38 kD
Observed band size: 65 kD
2. Blank control: U937.
Primary Antibody (green line): Rabbit Anti-CD244 antibody (TMAB-10451)
Dilution: 1 μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-PE
Dilution: 1 μg /test.
Protocol
The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
3. Sample: 293T (Human) Cell Lysate at 30 μg
Primary: Anti-phospho-CD244 (Tyr271) (TMAB-10451) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 38 kD
Observed band size: 70 kD

WB: 1:500-2000; FCM: 1μg/Test

Polyclonal

KLH conjugated Synthesised phosphopeptide: human CD244 around the phosphorylation site of Tyr271

Human

CD244

Natural killer cell receptor 2B4

Modulate other receptor-ligand interactions to enhance leukocyte activation.