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Anti-Phospho-CCNE1 (Thr77) Antibody (8N839)
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Catalog No. TMAC-03205
Alias p-CCNE1 (Thr77), p-CCNE1 (T77), CCNE1 (p-Thr77), CCNE1 (p-T77)
Anti-Phospho-CCNE1 (T77) Antibody (8N839) is a Rabbit antibody targeting Phospho-CCNE1 (T77). Anti-Phospho-CCNE1 (T77) Antibody (8N839) can be used in WB,ICC/IF,IHC.
Anti-Phospho-CCNE1 (Thr77) Antibody (8N839)
Anti-Phospho-CCNE1 (Thr77) Antibody (8N839)
😃Good
Catalog No. TMAC-03205Alias p-CCNE1 (Thr77), p-CCNE1 (T77), CCNE1 (p-Thr77), CCNE1 (p-T77)
Anti-Phospho-CCNE1 (T77) Antibody (8N839) is a Rabbit antibody targeting Phospho-CCNE1 (T77). Anti-Phospho-CCNE1 (T77) Antibody (8N839) can be used in WB,ICC/IF,IHC.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $298 | 7-10 days | |
| 100 μL | $498 | 7-10 days |
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| Description | Anti-Phospho-CCNE1 (Thr77) Antibody (8N839) is a Rabbit antibody targeting Phospho-CCNE1 (Thr77). Anti-Phospho-CCNE1 (Thr77) Antibody (8N839) can be used in WB,ICC/IF,IHC. |
| Synonyms | p-CCNE1 (Thr77), p-CCNE1 (T77), CCNE1 (p-Thr77), CCNE1 (p-T77) |
| Ig Type | IgG |
| Clone | 8N839 |
| Reactivity | Human |
| Verified Activity | 1. Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Phospho-Cyclin E1 (T77) antibody. Counter stained with hematoxylin. 2. Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Phospho-Cyclin E1 (T77) antibody. Counter stained with hematoxylin. 3. ICC staining Phospho-Cyclin E1 (T77) in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 4. ICC staining Phospho-Cyclin E1 (T77) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 5. ICC staining Phospho-Cyclin E1 (T77) in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
| Application | |
| Recommended Dose | WB: 1:1000; IHC: 1:50-200; ICC/IF: 1:50-200 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | Cyclins were first identified in invertebrates as proteins that oscillate dramatically through the cell cycle. These proteins have been well conserved through evolution and play a critical role in regulation of cell division. cyclin E, along with the three cyclin D proteins and cyclin C, has been shown to represent a putative G1 cyclin on the basis of its cyclic pattern of mRNA expression, with maximal levels being detected near the G1/S boundary. cyclin E has been found to be associated with the transcription factor E2F in a temporally regulated manner. The cyclin E/E2F complex is detected primarily during the G1 phase of the cell cycle and decreases as cells enter S phase. E2F is known to be a critical transcription factor for expression of several S phase specific proteins. |
| Conjucates | Unconjugated |
| Others Formats | Phospho |
| Immunogen | A synthesized phosphopeptide: human Cyclin E1 around the phosphorylation site of Thr77 |
| Antigen Species | human |
| Uniprot ID |
| Molecular Weight | Theoretical: 48 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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