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Anti-Phospho-CCNB1 (Ser147) Polyclonal Antibody is a Rabbit antibody targeting Phospho-CCNB1 (Ser147). Anti-Phospho-CCNB1 (Ser147) Polyclonal Antibody can be used in IF,IHC,WB.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $218 | 7-10 days | 7-10 days | |
| 100 μL | $316 | 7-10 days | 7-10 days |
| Description | Anti-Phospho-CCNB1 (Ser147) Polyclonal Antibody is a Rabbit antibody targeting Phospho-CCNB1 (Ser147). Anti-Phospho-CCNB1 (Ser147) Polyclonal Antibody can be used in IF,IHC,WB. |
| Synonyms | p-CCNB1 (Ser147), p-CCNB1 (S147), CCNB1 (p-Ser147), CCNB1 (p-S147) |
| Ig Type | IgG |
| Reactivity | Human |
| Verified Activity | 1. Western blot analysis of extracts from MDA cells untreated or treated with Anisomycin using Cyclin B1 (phospho-Ser147) Antibody TMAC-01034. 2. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Cyclin B1 (Phospho-Ser147) Antibody TMAC-01034 (left) or the same antibody preincubated with blocking peptide (right). 3. Immunofluorescence staining of methanol-fixed Hela cells using Cyclin B1 (phospho-Ser147) Antibody TMAC-01034. ![]() ![]() ![]() |
| Application | |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Construction | Polyclonal Antibody |
| Purification | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| Appearance | Liquid |
| Formulation | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Research Background | The protein encoded by Cyclin B1 is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. |
| Conjucates | Unconjugated |
| Others Formats | Phospho |
| Immunogen | Peptide sequence around phosphorylation site of Serine 147 (A-F-S(p)-D-V) derived from Human Cyclin B1 |
| Antigen Species | human |
| Uniprot ID |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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