Shopping Cart
Remove All
Your shopping cart is currently empty
Anti-Phospho-Caspase-9 (Ser196) Polyclonal Antibody
Copy Product Info
😃Good
Catalog No. TMAB-10443
Anti-Phospho-Caspase-9 (Ser196) Polyclonal Antibody is a Rabbit antibody targeting Phospho-Caspase-9 (Ser196). Anti-Phospho-Caspase-9 (Ser196) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.
Anti-Phospho-Caspase-9 (Ser196) Polyclonal Antibody
Anti-Phospho-Caspase-9 (Ser196) Polyclonal Antibody
Copy Product Info😃Good
Catalog No. TMAB-10443
Anti-Phospho-Caspase-9 (Ser196) Polyclonal Antibody is a Rabbit antibody targeting Phospho-Caspase-9 (Ser196). Anti-Phospho-Caspase-9 (Ser196) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $220 | 7-10 days | 7-10 days | |
| 100 μL | $374 | 7-10 days | 7-10 days | |
| 200 μL | $528 | 7-10 days | 7-10 days |
Add to Cart
Add to Quotation
In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.
Resource Download
Product Introduction
Bioactivity
Antigen Details
Chemical Properties
Stability & Storage
| Description | Anti-Phospho-Caspase-9 (Ser196) Polyclonal Antibody is a Rabbit antibody targeting Phospho-Caspase-9 (Ser196). Anti-Phospho-Caspase-9 (Ser196) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF. |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Sample: Brain (Rat) Lysate at 40 μg Kidney (Rat) Lysate at 40 μg Primary: Anti-Phospho-Caspase-9 (Ser196) (TMAB-10443) at 1/300 dilution Secondary: HRP conjugated Goat-Anti-rabbit IgG at 1/5000 dilution Predicted band size: 35 kD Observed band size: 35 kD 2. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Phospho-Caspase-9 (Ser196)) Polyclonal Antibody, Unconjugated (TMAB-10443) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. 3. Sample: Heart (Rat) Lysate at 40 μg Heart (Mouse) Lysate at 40 μg Hela (Human) Cell Lysate at 30 μg HepG2 (Human) Cell Lysate at 30 μg Primary: Anti-Phospho-Caspase-9 (Ser196) (TMAB-10443) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 46-51/35/37 kD Observed band size: 51/37 kD 4. HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (Phospho-Caspase-9 (Ser196)) polyclonal Antibody, Unconjugated (TMAB-10443) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei. |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Tissue Specificity | Ubiquitous, with highest expression in the heart, moderate expression in liver, skeletal muscle, and pancreas. Low levels in all other tissues. Within the heart, specifically expressed in myocytes. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | Caspase 9 (also known as ICE like apoptotic protease 6 (ICE LAP6), apoptotic protease Mch6, and apoptotic protease activating factor 3 (Apaf3)) is a member of the peptidase family C14 that contains a CARD domain. This caspase is active as a heterotetramer and has been reported to have two isoforms. ProCaspase 9 has been reported to be approximately 47 kD. This caspase is present in the cytosol and, upon activation, translocates to the mitochondria. Caspase 9 is involved in the caspase activation cascade responsible for apoptosis execution and cleaves/activates Caspase 3 and Caspase 6. Caspase 9 is inhibited by the dominant negative isoform, BclXL, cIAP1, cIAP2, XIAP, and Livin. This caspase becomes activated when recruited to Apaf1/cytochrome c complex, and following cleavage by Apaf1, granzyme B, Caspase 3, possibly Caspase 8 and Caspase 10 into large p37 and small p10 subunits. Caspase 9 intereacts with BIRC7 and has been shown to cleave PARP and vimentin. |
| Immunogen | KLH conjugated Synthesised phosphopeptide: human CASP9 around the phosphorylation site of Ser196 |
| Antigen Species | Human |
| Gene Name | CASP9 |
| Gene ID | |
| Protein Name | Caspase-9 |
| Uniprot ID | |
| Function | Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP).
Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9. |
| Molecular Weight | Theoretical: 35 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
Calculator
Preview
Related Tags: buy Anti-Phospho-Caspase-9 (Ser196) Polyclonal Antibody | purchase Anti-Phospho-Caspase-9 (Ser196) Polyclonal Antibody | Anti-Phospho-Caspase-9 (Ser196) Polyclonal Antibody cost | order Anti-Phospho-Caspase-9 (Ser196) Polyclonal Antibody | Anti-Phospho-Caspase-9 (Ser196) Polyclonal Antibody molecular weight
Generate Quote
Catalog No.: Cas No.:
Add
| Size | Quantity | Unit Price | Amount | Operation |
|---|
Generate Quote
- TOP
Feedback
Hello! How can I help you today? 
Copyright © 2015-2026 TargetMol Chemicals Inc. All Rights Reserved.