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Anti-Phospho-CASP9 (Thr125) Polyclonal Antibody 2
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Catalog No. TMAC-00573
Alias p-CASP9 (Thr125), p-CASP9 (T125), CASP9 (p-Thr125), CASP9 (p-T125)
Anti-Phospho-CASP9 (Thr125) Polyclonal Antibody is a Rabbit antibody targeting Phospho-CASP9 (Thr125). Anti-Phospho-CASP9 (Thr125) Polyclonal Antibody can be used in IHC,WB.
Anti-Phospho-CASP9 (Thr125) Polyclonal Antibody 2
Anti-Phospho-CASP9 (Thr125) Polyclonal Antibody 2
😃Good
Catalog No. TMAC-00573Alias p-CASP9 (Thr125), p-CASP9 (T125), CASP9 (p-Thr125), CASP9 (p-T125)
Anti-Phospho-CASP9 (Thr125) Polyclonal Antibody is a Rabbit antibody targeting Phospho-CASP9 (Thr125). Anti-Phospho-CASP9 (Thr125) Polyclonal Antibody can be used in IHC,WB.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $217 | 7-10 days | |
| 100 μL | $317 | 7-10 days |
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| Description | Anti-Phospho-CASP9 (Thr125) Polyclonal Antibody is a Rabbit antibody targeting Phospho-CASP9 (Thr125). Anti-Phospho-CASP9 (Thr125) Polyclonal Antibody can be used in IHC,WB. |
| Synonyms | p-CASP9 (Thr125), p-CASP9 (T125), CASP9 (p-Thr125), CASP9 (p-T125) |
| Ig Type | IgG |
| Reactivity | Human |
| Verified Activity | 1. Western blot analysis of extracts from HeLa cells treated with TNF using Caspase 9 (Phospho-Thr125) Antibody TMAC-00573. The lane on the right is treated with the antigen-specific peptide. 2. Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using Caspase 9 (Phospho-Thr125) antibody TMAC-00573 (left) or the same antibody preincubated with blocking peptide (right). |
| Application | |
| Recommended Dose | WB: 1:500-1000; IHC: 1:50-100 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Construction | Polyclonal Antibody |
| Purification | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| Appearance | Liquid |
| Formulation | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Research Background | Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP). Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9. |
| Conjucates | Unconjugated |
| Others Formats | Phospho |
| Immunogen | Peptide sequence around phosphorylation site of threonine 125 (P-E-T(p)-P-R) derived from Human Caspase 9 |
| Antigen Species | human |
| Uniprot ID |
| Molecular Weight | Actual: 47 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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