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Anti-Phospho-CASP9 (Thr125) Polyclonal Antibody is a Rabbit antibody targeting Phospho-CASP9 (Thr125). Anti-Phospho-CASP9 (Thr125) Polyclonal Antibody can be used in WB,IHC.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $217 | 7-10 days | |
100 μL | $317 | 7-10 days |
Description | Anti-Phospho-CASP9 (Thr125) Polyclonal Antibody is a Rabbit antibody targeting Phospho-CASP9 (Thr125). Anti-Phospho-CASP9 (Thr125) Polyclonal Antibody can be used in WB,IHC. |
Ig Type | IgG |
Reactivity | Human |
Application | |
Recommended Dose | WB: 1:500-1000; IHC: 1:50-100 |
Antibody Type | Polyclonal |
Host Species | Rabbit |
Construction | Polyclonal Antibody |
Purification | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
Appearance | Liquid |
Formulation | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Research Background | Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP). Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9. |
Conjucates | Unconjugated |
Others Formats | Phospho |
Immunogen | Peptide sequence around phosphorylation site of threonine 125 (P-E-T(p)-P-R) derived from Human Caspase 9 |
Antigen Species | human |
Uniprot ID |
Molecular Weight | Actual: 47 kDa. |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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